Inhibition of growth and proliferation of Hep-2 cells by targeting hTERT gene using small interference RNA technology.
- Author:
Haiying JIA
1
,
2
;
Tao ZHANG
;
Zhi TANG
;
Jiqun WANG
Author Information
1. Department of Otolaryngology Head and Neck Surgery, First Affiliated Hopsital, Ji'nan University, Guangzhou, China. jiahaiying79@
2. com
- Publication Type:Journal Article
- MeSH:
Carcinoma, Squamous Cell;
genetics;
Cell Line, Tumor;
Gene Targeting;
Genetic Vectors;
Humans;
Laryngeal Neoplasms;
genetics;
RNA;
genetics;
RNA, Messenger;
genetics;
RNA, Small Interfering;
Telomerase;
genetics;
Transfection
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(7):308-310
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the inhibition of hTERT gene to Hep-2 cells of laryngeal neoplasms.
METHOD:siRNA-hTERT mRNA was designed. Hep-2 cells, cultured in vitro, were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase (RT-PCR), MTT assay, and morphology.
RESULT:1. siRNA were transfected into Hep-2 cells successfully; 2. RT-PCR showed the exogenous hTERT was knocked down 70% by siRNA; 3. The morphology and the MTT result showed the hTERT siRNA to be able effectively to suppress the Hep-2 cell multiplication, the suppression rate achieves 65%.
CONCLUSION:siRNA target to hTERT can remarkably suppress the proliferation of Hep-2 cell.
