Interleukin-1beta induces MUC2/MUC5B gene expression in human nasal epithelial cells.
- Author:
Xuekun HUANG
1
;
Yuan LI
;
Tao WANG
;
Peng LI
;
Xian LIU
;
Hong LIU
;
Wiejie XU
Author Information
1. Department of Otorhinolaryngology, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, China.
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Epithelial Cells;
drug effects;
metabolism;
Humans;
Interleukin-1beta;
pharmacology;
Mucin-2;
genetics;
metabolism;
Mucin-5B;
genetics;
metabolism;
Nasal Mucosa;
cytology;
drug effects;
metabolism;
RNA, Messenger;
genetics;
Up-Regulation
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2010;24(14):632-635
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To demonstrate the effects of IL-1beta on MUC2/MUC5B gene expression in cultured human nasal epithelial cells.
METHOD:In passage-2 cultured human nasal epithelial cells, the mRNA levels of MUC2/MUC5B gene expression induced by IL-1 beta were determined by fluorescent quantitative RT-PCR.
RESULT:MUC2/MUC5B mRNAs were detected after 24 h of exposure to IL-1beta. MUC2 mRNA levels in IL-1 beta treatment [(39.26 +/- 6.10) x 10(4) copy/microg] were significantly higher than control [(5.70 +/- 4.16) x 10(4) copy/microg] (P < 0.01). MUC5BmRNA levels in IL-1beta treatment [(5.7 +/- 2.06) x 10(5) copy/microg] were significantly higher than control [(1.11 +/- 0.72) 10(5) copy/microg] (P < 0.05).
CONCLUSION:IL-1 beta increased MUC2/MUC5B mRNA levels in human nasal epithelial cells. These results suggest that IL-1beta may enhance mucin gene expression in cultured human nasal epithelial cells.
