The expression and significance of miRNA-324-3p and WNT2B in nasopharyngeal carcinoma.
- Author:
Chao LIU
;
Guo LI
;
Yong LIU
;
Zhongwu SU
;
Shuling REN
;
Tengbo DENG
;
Yongquan TIAN
;
Yuanzhen QIU
- Publication Type:Journal Article
- MeSH:
Carcinoma;
Glycoproteins;
genetics;
metabolism;
Humans;
Lymphatic Metastasis;
MicroRNAs;
metabolism;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms;
metabolism;
Neoplasm Proteins;
metabolism;
RNA, Messenger;
metabolism;
Wnt Proteins;
genetics;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(21):1676-1684
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression and significance of miRNA-324-3p and its target gene WNT2B in tissue specimens of nasopharyngeal carcinoma (NPC) specimens.
METHOD:qRT-PCR was used to detect the expression of miRNA-324-3p and WNT2B mRNA, and Western blot was applied to assay the expression of WNT2B protein in 39 cases of NPC specimens and 21 cases of non-carcinoma epithelium. The relationship between their expression levels and clinicopathological characteristics and their correlation with clinical pathological parameters was analyzed.
RESULT:The expression of miRNA-324-3p was significantly down-regulated decreased but WNT2B mRNA/protein increased obviously in NPC specimens (P < 0.01). A negative correlation between miRNA-324-3p and WNT2B was spotted (P < 0.05). The expression levels of these markers were closely correlated with T stage, clinic stage and cervical lymph node metastasis (P < 0.05).
CONCLUSION:The loss of miRNA-324-3p and ectopic WNT2B might co-induce the initiation and progression of NPC.
