Correlative study of nuclear factor-kappa B subunit p50 activity and IL-4 gamma-IFN expression in nasal polyps.
- Author:
Yunqiu LI
1
,
2
;
Yong FENG
;
Jihua WANG
;
Xuping XIAO
Author Information
1. Department of Otolaryngology, Hunan Province People's Hospital, Changsha, 410002, China. entliyunqiu@
2. com
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Case-Control Studies;
Female;
Humans;
Interferon-gamma;
metabolism;
Interleukin-4;
metabolism;
Male;
Middle Aged;
NF-kappa B p50 Subunit;
metabolism;
Nasal Polyps;
metabolism;
pathology;
RNA, Messenger;
genetics;
Young Adult
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2008;22(5):211-214
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the possible regulation mechanism of nuclear factor-kappa B subunit p50 on Th1/Th2 cytokine in nasal polyps.
METHOD:The expression of IL-4, gamma-IFN and p50 were examined by the method of immunohistochemistry staining in 40 cases of nasal polyps and 20 normal subjects, and the linear correlations between activity of p50 and IL-4, gamma-IFN were evaluated.
RESULT:The expression of p50 mainly distribute in the cytoplasm of epithelia, inflammatory cell and the epithelia of gland. The expression of IL-4 and the activity of p50 was increased significantly in nasal polyps (all P<0.01). But the expression of gamma-IFN had no significant difference between the groups (P>0.05). Pearson correlation analysis demonstrated that p50 nuclear-presented closely correlation with IL-4 level, but not gamma-IFN. The correlation coefficient was 0.70 for p50 and IL-4 (P<0.01), 0.14 for p50 and gamma-IFN (P>0.05).
CONCLUSION:The activity of p50 in nasal polyps is increased significantly. The activation of p50 may play an important role in the imbalance of Th1/Th2-typed cytokines by upregulating the level of IL-4.
