FA-MNP-MMP-9-ASODN complex construction and the study of its FA molecular targeting ability.
- Author:
Tao LIU
1
;
Minqiang XIE
;
Dong MA
;
Yiming XU
;
Hongzheng ZHANG
;
Tao ZHANG
Author Information
1. Department of Otolaryngology Head and Neck Surgery, People's Hospital of Jiangxi Province, Nanchang, 330006, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Folic Acid;
genetics;
Genetic Vectors;
Matrix Metalloproteinase 9;
genetics;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Nanocomposites;
Neoplasm Transplantation;
Oligonucleotides, Antisense;
genetics;
Transfection
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2013;27(11):593-597
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To construct FA targeted magnetic nanocomplex (FA-MNP-MMP-9-ASODN) loading matrix metalloproteinase 9 (MMP-9) antisense oligonucleotide (ASODN) and evaluate its targeting capacity and efficiency of gene transfection to folate receptor (FR) positive NPC.
METHOD:FA-MNP-MMP-9-ASODN was constructed by MMP-9-ASODN coupling with FA-MNP prepared by our research team through the aldehyde-ammonia condensation reaction. To analyze the feasibility of ASODN coupling with nanocarrier agarose gel electrophoresis. Two kinds of HNE-1 and CNE-2 cells and implanted tumors phagocytosis of FA-MNP-MMP-9-ASODN were observed by MRI on tumor-bearing nude mice, iron staining and TEM. To analyze gene transfection of the vector by observing FITC in the cell.
RESULT:The electrophoresis results revealed ASODN successfully coupling with FA-MNP. HNE-1 cell can effectively ingest the nanocomposite,with more FITC in the cell, but CNE-2 cell had not uptake for the nanocomposite, with no FITC in the cell. By comparing with CNE-2 tumor, HNE-1 tumor also can efficiently swallow the nanocomposite.
CONCLUSION:FA-MNP-MMP-9-ASODN nanocomplex is constructed successfully with good FA targeting ability and gene transfection.
