Quantitative analysis of interleukin-5 mRNA and protein in nasal polyps.
- Author:
Anbin ZHAO
;
Hua WANG
;
Hao WU
;
Yongguo YANG
;
Haiyan XU
;
Daxin WANG
- Publication Type:Journal Article
- MeSH:
Female;
Humans;
Interleukin-5;
genetics;
metabolism;
Male;
Nasal Polyps;
metabolism;
RNA, Messenger;
genetics;
Turbinates;
metabolism
- From:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
2014;28(14):1053-1056
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the expression of intedeukin-5 (IL-5) mRNA and protein in Nasal polyps and in the inferior turbinate, and to explore the relationship between the expression and the Clinical features.
METHOD:Real time fluorescent quantitative PCR(RT-qPCR) and immunohistochemical staining were used to detect the expression of intedeukin-5 (IL-5) mRNA and protein in nasal polyps of 24 cases and in inferior turbinate of 15 cases.
RESULT:The expression of IL-5 mRNA in Nasal polyps was 7.52 times higher than that in the inferior turbinate (P < 0.01). The expression rate of IL-5 protein in Nasal polyps was 79.17%, significantly higher than that in the inferior turbinate (26.67%) (chi2 = 10.52, P < 0.01). The differece of expression of IL-5 mRNA was not associated with the sexual distinction, unilateral or bilateral nasal polyps and primary or recurrent nasal polyps (P > 0.01), but was associated with the single or multiple nasal polyps, nasal polyps with or without allergic rhinitis and/or asthma. The differece of expression of IL-5 protein was not associated with the sexual distinction, unilateral or bilateral nasal polyps, primary or recurrent nasal polyps, nasal polyps with or without allergic rhinitis and/or asthma, but was associated with the single and multiple nasal polyps.
CONCLUSION:The high expression of IL-5 mRNA and protein is closely related with the formation edema and development of nasal polyps. Some clinical features of Nasal polyps related to the expression level of IL-5 in nasal polyps.
