Screening and identification of polypeptides targeting hepatitis B virus HBP-TP protein

VernacularTitle:靶向乙型肝炎病毒HBP-TP蛋白的多肽筛选与鉴定
Author: Juan DAI ¹ ; Fang PING ; Xun YANG ; Xuefei CAI
Author Information

1. 重庆医科大学
Keywords: Hepatitis B virus; HBP-TP; Bacteriophage display library
From: Chinese Journal of Microbiology and Immunology 2019;39(1):12-17
CountryChina
Language:Chinese
Abstract: Objective To identify specific peptides binding to hepatitis B virus ( HBV) DNA pol-ymerase TP region ( HBP-TP) and to study their effects on HBV replication. -ethods The phage polypep-tide display library was screened using the HBP-TP recombinant protein expressed in Escherichia coli as a substrate to obtain the specific phage, the polypeptide region of which was then sequenced. Polypeptide se-quences with a repetition rate greater than 10％ were counted and commercially synthesized. Changes in HBV replication were detected after treatment of HBV-expressing cells with synthetic peptides as drugs. Re-sults Eight peptides targeting HBP-TP recombinant protein were screened out from the phage display librar-y. One of the peptides was found to have a significant inhibitory effect on HBV replication at cellular level. Conclusions A HBP-TP protein-targeting polypeptide with an inhibitory effect on HBV replication was ob-tained.