Effect of endocrine gland-derived vascular endothelial growth factor on the proliferation and apoptosis of pancreatic cancer cells
10.3760/cma.j.issn.1007-8118.2019.04.010
- VernacularTitle:内分泌腺来源的血管内皮生长因子对胰腺癌细胞增殖和凋亡的影响
- Author:
Xiongwei FAN
1
;
Maojun WANG
;
Haitao YANG
;
Feng WANG
;
Qi WANG
Author Information
1. 宁夏医科大学附属吴忠市人民医院普外科
- Keywords:
Pancreatic neoplasms;
EG-VEGF;
Proliferation;
Apoptosis;
Signal transduction
- From:
Chinese Journal of Hepatobiliary Surgery
2019;25(4):283-287
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of endocrine gland-derived vascular endothelial growth factor(EG-VEGF) on the proliferation and apoptosis of pancreatic cancer cells and on the regulation of PI3K/AKT signaling pathway.Methords The expression of EG-VEGF and its receptor-VEGFR-2 in pancreatic cancer tissues and adjacent normal tissues was detected by PCR.The effect of EG-VEGF on proliferation of human pancreatic cancer cells was detected by MTT assay.The effect of EG-VEGF on the apoptosis of human pancreatic cancer cells was detected by flow cytometry.Western lot was used to detect the changes of related proteins expression in PI3K/AKT signaling pathway after EG-VEGF treatment.Results The expression (3.08±0.30,2.17±0.16 respectively) of EG-VEGF and VEGFR-2 in pancreatic cancer tissues was significantly higher than those (1.55±0.73,0.54±0.34 respectively) in adjacent tissues (both P＜ 0.05).MTT and flow cytometry data showed that EG-VEGF could promote the proliferation of pancreatic cancer cells and inhibit cell apoptosis.Western blot showed that EG-VEGF promoted AKT phosphorylation and activated PI3K/AKT signaling pathway.Conclusion EG-VEGF is highly expressed in pancreatic cancer tissue,and can activate PI3K/AKT signaling pathway to promote cell proliferation and inhibit cell apoptosis.
