Mir-361-5p enhances radiosensitivity of osteosarcoma cells by directly downregulating FOXM1
10.3760/cma.j.issn.1004-4221.2019.05.012
- VernacularTitle:miR-361-5p负调控靶基因FoxM1增加骨肉瘤细胞放射敏感性研究
- Author:
Shuai DING
1
;
Yanzheng GAO
;
Guangquan ZHANG
;
Shulian CHEN
;
Chen CAO
;
Baiyu LI
Author Information
1. 河南省人民医院脊柱脊髓外科
- Keywords:
Osteosarcoma cell line;
Gene expression regulation;
Radiosensitivity
- From:
Chinese Journal of Radiation Oncology
2019;28(5):378-381
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of microRNA361-5p on radiosensitivity of osteosarcoma cells and its downstream regulatory mechanisms.Methods The radioresistant osteosarcoma cell line HOS-R was constructed and the expression of microRNA-361-Sp in HOS and HOS-R cells was detected by RT-qPCR.The survival rate and apoptosis rate were detected by clone formation assay and flow cytometry in HOS-R cells treated with up-regulated or down-regulated of miR-361-Sp,or FOXM1 depletion.Dual fluorescent luciferase reporter and western blot assays were used to measure the relationship between miR-361-5p and FOXM1.he effects of miR-361-5p on radiosensitivity of osteosarcoma cells were determined by cloning formation assay and flow cytometry.Results RT-qPCR results showed that miR-361-5p was low expressed in HOS-R cells.Colony formation and flow cytometry assays demonstrated that overexpression of miR-361-5p significantly decreased the survival rate and increased the apoptosis rate of HOS-R cells.In contrast,FOXM1 downregulation inhibited cell survival rate and induced apoptosis.Moreover,miR-361-5p negatively regulated FOXM1 expression.Besides,FOXM1 overexpression attenuated the miR-361-5p upregulation-mediated promotion of radiosensitivity in HOS-R cells.Conclusion miR-361-5p was involved in the radiosensitivity of osteosarcoma cells by inhibiting FOXM1.
