SDF-1α/CXCR4 axis promotes migration and invasion of pancreatic cancer cells through inducing epithelial-mesenchymal transition
10.3969/j.issn.1000-4718.2019.02.014
- VernacularTitle:SDF-1α/CXCR4轴通过诱导胰腺癌上皮-间充质转化促进肿瘤迁移和侵袭
- Author:
Ruo-Meng LI
1
;
Jin-Mao ZOU
;
Ya-Qing LI
;
Shao-Jie CHEN
;
Guo-Da LIAN
;
Yin-Ting CHEN
;
Hong SU
;
Kai-Hong HUANG
Author Information
1. 中山大学孙逸仙纪念医院消化内科
- Keywords:
Pancreatic cancer;
SDF-1/CXCR4 axis;
Epithelial-mesenchymal transition
- From:
Chinese Journal of Pathophysiology
2019;35(2):273-279
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the role of SDF-1α/CXCR4 axis in pancreatic cancer cell migration and invasion.METHODS:The mRNA expression of CXCR4 in 4 pancreatic cancer cell lines was detected by RT-qPCR.The migration and invasion abilities of PANC-1 cells with the axis activated by exogenous SDF-1αor inhibited by CXCR4 inhibitor AMD3100 were detected by Transwell assays.The cell viability was measured by MTS assay.The protein expression of the epithelial-mesenchymal transition (EMT) -related molecules in the cells treated with exogenous SDF-1αor AMD3100 was determined by Western blot.RESULTS:All of the 4 pancreatic cancer cell lines expressed CXCR4 mRNA, while the PANC-1 cell line expressed the most.Exogenous SDF-1αpromoted the migration and invasion abilities of PANC-1 cells, which was inhibited by AMD3100.The PANC-1 cells treated with exogenous SDF-1αfor 72 h grew faster, while SDF-1αcombined with AMD3100 made little significance to the viability of PANC-1 cells.Exogenous SDF-1αinduced EMT of PANC-1 cells by up-regulating the expression of SNAIL and TWIST, and AMD3100 reversed this effect.CONCLUSION:SDF-1α/CXCR4 axis enhances the migration and invasion abilities of pancreatic cancer cells through inducing EMT.
