Expression of piR-9994 in gastric carcinoma and its correlation with PIWIL4
10.13315/j.cnki.cjcep.2019.02.007
- VernacularTitle:胃癌组织中piR-9994的表达及其与PIWIL4表达的相关性
- Author:
Yan XIA
1
;
Xian-Dong LIN
;
Dan HU
;
He-Jun ZHANG
;
Jie-Qiong LIN
;
Gang CHEN
;
Xiong-Wei ZHENG
Author Information
1. 福建省肿瘤医院/福建医科大学附属肿瘤医院病理科
- Keywords:
gastric neoplasms;
piR-9994;
PIWIL4;
immunohistochemistry;
qRT-PCR
- From:
Chinese Journal of Clinical and Experimental Pathology
2019;35(2):155-160
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the relationship between the expression level of piR-9994 in gastric cancer and its clinical pathological features,and to analyze its correlation with PIWIL4 expression. Methods Express of piR-9994 and PIWIL4 in 76 cases of human gastric cancer tissue with different clinical stage and differentiated degree and matching adjacent tissue were detected by qRT-PCR and immunohistochemistry,respectively. Results The expression of piR-9994 in gastric cancer was 2. 3 times higher than that in paracancerous tissues (P = 0. 002 2) . The expression of piR-9994 in stage Ⅲ + Ⅳ gastric cancer was 3. 5 times higher than that in stage Ⅰ + Ⅱ (P = 0. 002) ,and the expression of piR-9994 in cancers with nerve invasion was 2. 5 times higher than that in cancers without invasion (P = 0. 036) . The positive expression of PIWIL4 in gastric cancer tissues was significantly higher than that in adjacent tissues (χ2 = 18. 346,P < 0. 001) ,and the expression of PIWIL 4 in stage Ⅲ +Ⅳ gastric cancer group was higher than that stage Ⅰ + Ⅱ gastric cancer group (χ2 = 8. 60,P = 0. 003) . There was a positive correlation between piR-9994 expression and PIWIL4 expression in gastric carcinoma (r = 0. 231,P < 0. 05) . Conclusion piR-9994 overexpression in gastric cancer tissues is closely related to tumor staging and nerve invasion,and piR-9994 may promote the occurrence and progression of gastric cancer by regulating PIWIL4 expression. piR-9994 may be a molecular markers for judging malignant degree and prognosis of gastric cancer.
