Study on effects and mechanism of interleukin 16 on pathogenesis of atherosclerosis
10.3969/j.issn.1673-4130.2019.02.003
- VernacularTitle:白细胞介素16对动脉粥样硬化的影响及其机制研究
- Author:
Wang GE
1
;
Bo WANG
;
Fenglian YAN
;
Guomei XU
;
Feng JIN
;
Qingqing ZHANG
;
Qun MA
;
Chuanping SI
Author Information
1. 青岛大学基础医学院免疫学系
- Keywords:
atherosclerosis;
atherosclerotic plaque;
interleukin 16
- From:
International Journal of Laboratory Medicine
2019;40(2):136-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression of interleukin 16 (IL-16) in atherosclerosis (AS) patients, and to study the roles of IL-16in the pathogenesis of AS.Methods Thirty AS patients in Affiliated Hospital of Jining Medical College from August 2015to August 2016were randomly selected as the case group and twenty-nine healthy subjects were selected as the healthy control group.Peripheral blood of the subjects were collected.IL-16levels were determined with enzyme-linked immunosorbent assay (ELISA).Reverse transcriptase-polymerase chain reaction was applied to analyze IL-16mRNA level.IL-16expression in the atherosclerotic plaque samples was detected with immunohistochemical analysis.IL-16expression in aortic atherosclerotic plaque of AS patients and atherosclerotic ApoE-/-mice were analyzed by immunohistochemical staining.The aortic plaque changes of AS mice injected intraperitoneally with recombinant IL-16were detected.Results Both IL-16protein levels and IL-16mRNA levels were higher in case group than those of healthy control group, the difference was statistically significant (P＜0.05).The IL-16mRNA was highly expressed in the atherosclerotic plaque.The aortic plaque area of the mice underwent IL-16intraperitoneal injection were decreased while the plaque stability increased.Conclusion IL-16levels elevated in both AS patients and AS mice, which suggested that IL-16might play aprotective role against AS.
