Mechanism of Aurora-A to promote chemoresistance in nasopharyngeal carcinoma
10.16066/j.1672-7002.2019.01.002
- VernacularTitle:Aurora-A促进鼻咽癌化疗抵抗的机制研究
- Author:
Xiaoqin FAN
1
;
Jian SONG
;
Yujie WANG
;
Hanwei WU
;
Lu LU
;
Guohui NIE
Author Information
1. 中山大学附属第一医院耳鼻咽喉科
- Keywords:
Nasopharyngeal Neoplasms;
Apoptosis;
Cisplatin;
chemotherapy resistance;
Aurora kinase A
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2019;26(1):5-8
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the mechanism of Aurora kinase A (Aurora-A) promoting cancer cell chemotherapy resistance in nasopharyngeal carcinoma. METHODS The expression of Aurora-A in nasopharyngeal carcinoma tissues and adjacent tissues were detected by Western bolt and Q-PCR. The highexpressing Aurora A cell line CNE2 was used to detected the cell apoptosis and the expression of key pathway marker protein after Aurora-A inhibitor VX680 and cisplatin treatment by using Flow cytometry and WB. RESULTS The expression of Aurora-A in nasopharyngeal carcinoma tissues was significantly higher than that in adjacent tissues. Comparing to normal nasopharyngeal cells NP69, Aurora-A was significantly highly expressed in all of nasopharyngeal carcinoma cells and was highest in CNE2. Inhibiton of Aurora-A increased the cell apoptosis and the expression of p-AKT, p21 and Cleaved-Caspase-3 after using cisplatin or the Aurora-A inhibitor VX680 treatment. CONCLUSION The results shown that Aurora-A confer chemoresistance to cisplatin treatment through p-AKT/p21/Cleaved-Caspase-3 pathway.
