MiR-182-5p Knockdown Targeting PTEN Inhibits Cell Proliferation and Invasion of Breast Cancer Cells
10.3349/ymj.2019.60.2.148
- Author:
Yue Sheng ZHAO
1
;
Wei Chao YANG
;
Hong Wei XIN
;
Ji Xia HAN
;
Su Gang MA
Author Information
1. Department of Breast Surgery, The Third Hospital Affiliated to Qiqihar Medical College, Qiqihar, China.
- Publication Type:Original Article
- Keywords:
Breast cancer;
miR-182-5p;
PTEN;
proliferation;
invasion
- MeSH:
Blotting, Western;
Breast Neoplasms;
Breast;
Carcinogenesis;
Cell Count;
Cell Proliferation;
Chromosomes, Human, Pair 10;
Computational Biology;
Female;
Heterografts;
Humans;
Immunoprecipitation;
Luciferases;
MicroRNAs;
Real-Time Polymerase Chain Reaction;
RNA
- From:Yonsei Medical Journal
2019;60(2):148-157
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Breast cancer (BC) is one of the most common malignant tumors, affecting a significant number of women worldwide. MicroRNAs (miRNAs) have been reported to play important roles in tumorigenesis. The aim of this study was to determine the roles of miR-182-5p in BC progression. MATERIALS AND METHODS: The expressions of miR-182-5p and phosphatase and tensin homolog deleted on chromosome 10 (PTEN) were measured in BC tissues and cells by quantitative real-time polymerase chain reaction or Western blot. Cell proliferation and invasion were detected by cell counting kit-8 assay and trans-well assay, respectively. The interaction between miR-182-5p and PTEN was probed by bioinformatics analysis, luciferase activity, and RNA immunoprecipitation. A murine xenograft model was established to investigate the role of miR-182-5p in BC progression in vivo. RESULTS: An abundance of miR-182-5p was noted in BC tissues and cells. High expression of miR-182-5p was associated with poor survival. Abrogation of miR-182-5p inhibited cell proliferation and invasion in BC cells. Interestingly, PTEN was indicated as a target of miR-182-5p, and its restoration reversed miR-182-5p-mediated promotion of proliferation and invasion of BC cells. Moreover, depletion of miR-182-5p suppressed tumor growth via up-regulating PTEN expression in the murine xenograft model. CONCLUSION: MiR-182-5p exhaustion blocked cell proliferation and invasion by regulating PTEN expression, providing a novel therapeutic avenue for treatment of BC.
