Effects of the cathepsin K inhibitor with mineral trioxide aggregate cements on osteoclastic activity
- Author:
Hee Sun KIM
1
;
Soojung KIM
;
Hyunjung KO
;
Minju SONG
;
Miri KIM
Author Information
- Publication Type:Original Article
- Keywords: Cathepsin K; Osteoclastogenesis; Osteoclast; Osteoblast; Root resorption
- MeSH: Acid Phosphatase; Alendronate; Calcium; Cathepsin K; Cathepsins; Cell Count; Cytokines; Dinoprostone; Enzyme-Linked Immunosorbent Assay; Interleukin-6; Interleukins; Miners; Necrosis; Osteoblasts; Osteoclasts; Pemetrexed; Polymerase Chain Reaction; RAW 264.7 Cells; Receptor Activator of Nuclear Factor-kappa B; Replantation; Root Resorption; Tooth
- From:Restorative Dentistry & Endodontics 2019;44(2):e17-
- CountryRepublic of Korea
- Language:English
- Abstract: OBJECTIVES: Root resorption is an unexpected complication after replantation procedures. Combining anti-osteoclastic medicaments with retrograde root filling materials may avert this resorptive activity. The purpose of this study was to assess effects of a cathepsin K inhibitor with calcium silicate-based cements on osteoclastic activity. METHODS: MC3T3-E1 cells were cultured for biocompatibility analyses. RAW 264.7 cells were cultured in the presence of the receptor activator of nuclear factor-kappa B and lipopolysaccharide, followed by treatment with Biodentine (BIOD) or ProRoot MTA with or without medicaments (Odanacatib [ODN], a cathepsin inhibitor and alendronate, a bisphosphonate). After drug treatment, the cell counting kit-8 assay and Alizarin red staining were performed to evaluate biocompatibility in MC3T3-E1 cells. Reverse-transcription polymerase chain reaction, tartrate-resistant acid phosphatase (TRAP) staining and enzyme-linked immunosorbent assays were performed in RAW 264.7 cells to determine the expression levels of inflammatory cytokines, interleukin (IL)-1β, IL-6, tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2). Data were analyzed by one-way analysis of variance and Tukey's post hoc test (p < 0.05). RESULTS: Biocompatibility results showed that there were no significant differences among any of the groups. RAW 264.7 cells treated with BIOD and ODN showed the lowest levels of TNF-α and PGE2. Treatments with BIOD + ODN were more potent suppressors of inflammatory cytokine expression (p < 0.05). CONCLUSION: The cathepsin K inhibitor with calcium silicate-based cement inhibits osteoclastic activity. This may have clinical application in preventing inflammatory root resorption in replanted teeth.
