Identification and HPLC Quantification of a Phytoecdysone and Three Phenolic Glycosides in Lamium takesimense Nakai
10.20307/nps.2018.24.4.241
- Author:
Agung NUGROHO
1
;
Kyoung Sik PARK
;
Dong Jin SEO
;
Hee Juhn PARK
Author Information
1. Department of Agro-industrial Technology, LambungMangkurat University, Banjarbaru 70714, Indonesia.
- Publication Type:Original Article
- Keywords:
Lamium takesimense Nakai;
Lamiaceae;
ecdysone;
rutin;
HPLC
- MeSH:
Acetic Acid;
Chromatography, High Pressure Liquid;
Ecdysone;
Ecdysterone;
Glycosides;
Lamiaceae;
Methods;
Phenol;
Plants;
Rutin
- From:Natural Product Sciences
2018;24(4):241-246
- CountryRepublic of Korea
- Language:English
-
Abstract:
The herbs of Lamium takesimense Nakai (Lamiaceae) is used to treat spasmodic and inflammatory disease. The four polar compounds, ecdysterone, isoacteoside, rutin and lamiuside C, were isolated and identified from the BuOH fraction of the L. takesimense MeOH extract. HPLC quantification was performed on a Capcell Pak C18 column (5 µm, 4.6 mm × 250 mm) with a gradient elution of H₂O and 0.05% acetic acid in MeOH. The HPLC method was validated in terms of linearity, sensitivity, stability, precision, and accuracy. The quantitative level in plant material was determined as the following order: lamiuside C (4, 3.75 mg/g dry weight) > ecdysterone (1, 1.93 mg/g) > isoacteoside (2, 1.32 mg/g) > rutin (3, 0.97 mg/g).
