Preliminary assessment of correlation between T-lymphocyte responses and control of porcine reproductive and respiratory syndrome virus (PRRSV) in piglets born after in-utero infection of a type 2 PRRSV
10.14405/kjvr.2018.58.1.9
- Author:
Sang Ho CHA
1
;
Carey BANDARANAYAKA-MUDIYANSELAGE
;
Chandima B BANDARANAYAKA-MUDIYANSELAGE
;
Dharani AJITHTHOS
;
Kyoung Jin YOON
;
Kathleen A GIBSON
;
Ji Eun YU
;
In Soo CHO
;
Stephen S LEE
;
Chungwon J CHUNG
Author Information
1. Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
- Publication Type:Original Article
- Keywords:
T-lymphocyte;
neutralizing antibodies;
porcine reproductive and respiratory syndrome virus
- MeSH:
Adaptive Immunity;
Antibodies;
Antibodies, Neutralizing;
Interferons;
Lung;
Lymph Nodes;
Lymphoid Tissue;
Macrophages, Alveolar;
Phenotype;
Plasma;
Porcine Reproductive and Respiratory Syndrome;
Porcine respiratory and reproductive syndrome virus;
T-Lymphocytes;
Viral Load
- From:Korean Journal of Veterinary Research
2018;58(1):9-16
- CountryRepublic of Korea
- Language:English
-
Abstract:
A preliminary study into the protective mechanisms of adaptive immunity against porcine reproductive and respiratory syndrome virus (PRRSV) in piglets (n = 9) born to a gilt challenged intranasally with a type-2 PRRSV. Immune parameters (neutralizing antibodies, CD3⁺CD4⁺, CD3⁺CD8⁺, CD3⁺CD4⁺CD8⁺ T-lymphocytes, and PRRSV-specific interferon (IFN)-γ secreting T-lymphocytes) were compared with infection parameters (macro- and microscopic lung lesion, and PRRSV-infected porcine alveolar macrophages (CD172α⁺PRRSV-N⁺ PAM) as well as with plasma and lymphoid tissue viral loads. Percentages of three T-lymphocyte phenotypes in 14-days post-birth (dpb) peripheral blood mononuclear cell (PBMC) had significant negative correlations with percentages of CD172α⁺PRRSV-N⁺ PAM (p < 0.05) as well as with macroscopic lung lesion (p < 0.01). Plasma and tissue viral loads had significant (p < 0.05) negative correlations with CD3⁺CD4⁺CD8⁺ T-lymphocyte percentage in PBMC. Frequencies of CD3⁺CD8⁺ and CD3⁺CD4⁺ T-lymphocytes in 14-dpb PBMC had significant negative correlations with of lymph node (p = 0.04) and lung (p = 0.002) viral loads. IFN-γ-secreting T-lymphocytes frequency had a significant negative correlation with gross lung lesion severity (p = 0.002). However, neutralizing antibody titers had no significant negative correlation (p > 0.1) with infection parameters. The results indicate that T-lymphocytes contribute to controlling PRRSV replication in young piglets born after in-utero infection.
