Gomisin G Suppresses the Growth of Colon Cancer Cells by Attenuation of AKT Phosphorylation and Arrest of Cell Cycle Progression
10.4062/biomolther.2018.054
- Author:
Sony MAHARJAN
1
;
Byoung Kwon PARK
;
Su In LEE
;
Yoongho LIM
;
Keunwook LEE
;
Younghee LEE
;
Hyung Joo KWON
Author Information
1. Center for Medical Science Research, College of Medicine, Hallym University, Chuncheon 24252, Republic of Korea. hjookwon@hallym.ac.kr
- Publication Type:Original Article
- Keywords:
Gomisin G;
Colon cancer;
AKT;
Apoptosis;
PARP;
Cell cycle
- MeSH:
Annexin A5;
Apoptosis;
Caspase 3;
Cell Cycle;
Colon;
Colonic Neoplasms;
Colorectal Neoplasms;
Cyclin D1;
Phosphorylation;
Prognosis;
Retinoblastoma;
Ribose
- From:Biomolecules & Therapeutics
2019;27(2):210-215
- CountryRepublic of Korea
- Language:English
-
Abstract:
Colorectal cancer is one of the leading causes of cancer related death due to a poor prognosis. In this study, we investigated the effect of Gomisin G on colon cancer growth and examined the underlying mechanism of action. We found that Gomisin G significantly suppressed the viability and colony formation of LoVo cells. Gomisin G reduced the phosphorylation level of AKT implying that Gomisin G suppressed the PI3K-AKT signaling pathway. Gomisin G also induced apoptosis shown by Annexin V staining and an increased level of cleaved poly-ADP ribose polymerase (PARP) and Caspase-3 proteins. Furthermore, Gomisin G remarkably triggered the accumulation of cells at the sub-G1 phase which represents apoptotic cells. In addition, the level of cyclin D1 and phosphorylated retinoblastoma tumor suppressor protein (Rb) was also reduced by the treatment with Gomisin G thus curtailing cell cycle progression. These findings show the suppressive effect of Gomisin G by inhibiting proliferation and inducing apoptosis in LoVo cells. Taken together, these results suggest Gomisin G could be developed as a potential therapeutic compound against colon cancer.
