Effects of Valproic Acid on the Survival of Human Tennon's Capsule Fibroblasts
10.3341/jkos.2018.59.11.1056
- Author:
See Eun LEE
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Daegu Catholic University School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
Fibroblast;
Flow cytometry;
Mitomycin C;
Valproic acid
- MeSH:
Apoptosis;
Cell Survival;
Fibroblasts;
Flow Cytometry;
Humans;
Mitomycin;
Tenon Capsule;
Valproic Acid
- From:Journal of the Korean Ophthalmological Society
2018;59(11):1056-1061
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effects of valproic acid on the survival of cultured human Tenon's capsule fibroblasts (HTFBs). METHODS: Primary cultured HTFBs were exposed to 0, 0.25, 0.5, and 1.0 mM valproic acid with or without 0, 1.0, 2.5 µg/mL mitomycin C, and incubated for 5 days. Cell survival was assessed using an MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay and the degree of apoptosis was assessed by flow cytometry using annexin-V/propidium iodide double staining. RESULTS: Valproic acid decreased the survival of HTFBs in a dose-dependent manner, and survival was further decreased by adding mitomycin C to valproic acid. Both valproic acid and mitomycin C induced apoptosis of HTFBs. Valproic acid induced less apoptosis than mitomycin C. CONCLUSIONS: Valproic acid decreased the cellular survival of HTFBs and induced apoptosis. The antiproliferative effects of valproic acid were further enhanced by the addition of mitomycin C.
