Clinical value assessment of chemiluminescence for urine asymmetric dimethylarginine
10.3760/cma.j.issn.1009-9158.2018.12.010
- VernacularTitle:化学发光法检测尿液非对称二甲基精氨酸的临床价值评估
- Author:
Zhenyong WANG
1
;
Guosong SHEN
;
Pingya HE
;
Maoyu LI
;
Gang LI
Author Information
1. 313000,浙江省湖州市妇幼保健院检验科
- Keywords:
Arginine;
Urinalysis;
Luminescence;
Chromatography;
high pressure liquid;
Hypertension,pregnancy-induced
- From:
Chinese Journal of Laboratory Medicine
2018;41(12):948-951
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the clinical application value of chemiluminescence detection of urine asymmetric dimethylarginine ( ADMA ) in pregnancy-induced hypertension ( PIH ) . Methods Collected the 24 h urine from 60 normal pregnancy women and 72 PIH pregnancy women who were admitted to Huzhou Maternal and Child Health Hospital from May 2014 to April 2015 by the case-control study , Determination of urine ADMA content by chemiluminescence ( CLIA ) and high performance liquid chromatography ( HPLC ) , the results between two assays were analyzed by the Rank sum test , receiver operating characteristic ( ROC) curve and pearson correlation analysis .Results Compared with the normal control group , the urine ADMA concentration in the PIH group was significantly increased by HPLC and CLIA, and the concentration of ADMA by CLIA in the PIH group was 68.18(57.25,81.55)μmol/L higher than that of the normal control group 30.11(22.69,42.97)μmol/L(Z=-8.139,P<0.001),and the concentration of ADMA by HPLC in the PIH group by HPLC was 71.11(57.65,82.89)μmol/L higher than that of the normal control group 28.11(21.06,42.99)μmol/L(Z=-8.356,P<0.001).The difference was statistically significant .The two methods of urine ADMA concentration were highly positively correlated with PIH blood pressure.The correlation coefficient r values were 0.746 and 0.763, respectively, the P values were 0.007 and 0.008 respectively.Conclusions CLIA can better detect the ADMA concentration in urine of pregnant women with PIH , and has a good clinical diagnosis ability .The ADMA concentration in urine is related to the blood pressure level of PIH .
