Effect of different concentrations of morphine on viability of cortical neuron and synaptic plasticity in newborn mice
10.3760/cma.j.issn.0254-1416.2018.10.009
- VernacularTitle:不同浓度吗啡对新生小鼠皮层神经元活力和突触可塑性的影响
- Author:
Yi REN
1
;
Weihua CUI
;
Shanshan WANG
;
Min ZENG
;
Ruquan HAN
Author Information
1. 100045,国家儿童医学中心 首都医科大学附属北京儿童医院麻醉科
- Keywords:
Morphine;
Neuronal plasticity;
Infant,newborn
- From:
Chinese Journal of Anesthesiology
2018;38(10):1190-1193
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different concentrations of morphine on the viability of cortical neurons and synaptic plasticity in newborn mice.Methods Cortical neurons isolated from male C57/BL/6 mice within 24 h after birth were seeded in 60-mm cultured dish (10 ml),24-well plates (500 ml) and 96-well plates (100 ml) at a density of 2×106,1×105 and 1.5×104 cells per plate/well,respectively,and cultured for 7 days.Neurons were then divided into 4 groups (n =6 each) using a random number table method:control group (group C),morphine 1.0 mmol/L group (group M1),morphine 10.0 mmol/L group (group M2),and morphine 100.0 mmol/L group (group M3).Neurons were incubated for 48 h with morphine 1.0,10.0 and 100.0 mmol/L in M1,M2 and M3 groups,respectively.MTT assay was used to measure the cell viability,immunofluorescence to measure the length of microtube-associated protein-2-labeled dendrites,and Western blot to detect the expression of caveolin-1 (Cav-1),growth-associated protein-43 (GAP-43),synapsin,synaptophysin Ⅰ and vesicle-associated membrane protein.Results Compared with group C,the length of dendrites was significantly prolonged,and the expression of Cav-1,GAP-43,synapsin,synaptophysin Ⅰ and vesicle-associated membrane protein was up-regulated in group M2,and the cell viability was significantly decreased,and the expression of Cav-1,GAP-43 and vesicle-associated membrane protein was up-regulated in group M3 (P＜ 0.05).Conclusion Morphine 10.0 mmol/L causes no damage to cortical neurons and enhances synaptic plasticity of neurons in newborn mice.
