Effect of 17β estradiol on propofol-induced long-term cognitive dysfunction in developing rats: the relationship with hippocampal Glu∕GABA
10.3760/cma.j.issn.0254-1416.2018.09.011
- VernacularTitle:17β雌二醇对丙泊酚致发育期大鼠远期认知功能障碍的影响:与海马Glu∕GABA的关系
- Author:
Xiaoqian WU
1
;
Jianli LI
;
Junfang RONG
Author Information
1. 河北省人民医院麻醉科
- Keywords:
Propofol;
Estradiol;
Cognition disorders;
Glutamic acid;
gamma-Aminobutyric acid;
Hippocampus
- From:
Chinese Journal of Anesthesiology
2018;38(9):1065-1068
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of 17βestradiol on propofol-induced long-term cogni-tive dysfunction in developing rats and the relationship with hippocampal glutamate ( Glu )∕γ-aminobutyric acid (GABA). Methods Sixty healthy male Sprague-Dawley rats, aged 7 days, weighing 11-18 g, were divided into 5 groups ( n=12 each) using a random number table method: dimethyl sulfoxide ( DM-SO) group, fat emulsion group (group F), 17β estradiol group (group E), propofol group (group P) and propofol plus 17β estradiol group ( group P+E) . 17β estradiol 600 μg∕kg was subcutaneously injected in group E, and the equal volume of DMSO was given instead in group DMSO. Propofol 75 mg∕kg was in-traperitoneally injected in group P, and the equal volume of fat emulsion was given instead in group F. 17βestradiol 600μg∕kg was subcutaneously injected and 30 min later propofol 75 mg∕kg was intraperitoneally in-jected in group P+E. Injection was performed once every 24 h for 7 consecutive days in each group. Morris water maze test was performed at 60 days of age. The rats were sacrificed after the end of Morris water maze test and hippocampi were removed for determination of Glu content ( by ultraviolet colorimetry method) andGABA content (using enzyme-linked immunosorbent assay) in hippocampal tissues. Glu∕GABA ratio was calculated. Results There was no significant difference in the escape latency, the number of crossing the original platform, percentage of time spent in target quadrant, Glu content or Glu∕GABA ratio between group DMSO, group F and group E (P>0. 05). There was no significant difference in GABA content a-mong the five groups ( P>0. 05) . Compared with group F, the escape latency was significantly prolonged, the number of crossing the original platform was reduced, and the percentage of time spent in target quad-rant, Glu content and Glu∕GABA ratio were decreased in group P (P<0. 05). Compared with group P, the escape latency was significantly shortened, the number of crossing the original platform was increased, and the percentage of time spent in target quadrant, Glu content and Glu∕GABA ratio were increased in group P+E ( P<0. 05) . Conclusion 17β estradiol can improve propofol-induced long-term cognitive dysfunction and the mechanism may be related to maintaining hippocampal Glu∕GABA balance in developing rats.
