Observation of ultrastructure of morphological relationship between neurons and astrocytes in hippocampi of pentylenetetrazol-kindled epileptic rats
10.3760/j.issn.2095-428X.2013.05.016
- VernacularTitle:戊四氮致(癎)大鼠海马神经元与星形胶质细胞间关系的超微结构观察
- Author:
Li LAN
1
;
Yi LAN
;
Li DUAN
;
Wei ZHANG
;
Xun JIANG
;
Bao-Xi WANG
Author Information
1. 第四军医大学唐都医院儿科
- Keywords:
Epilepsy;
Astrocyte;
Neuron;
Gap junction;
Hemichannel;
Electron microscope
- From:
Chinese Journal of Applied Clinical Pediatrics
2013;28(5):373-376
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe ultrastructure of the morphological relationship between neuron and astrocytes in hippocampi of pentylenetetrazol (PTZ)-kindled epileptic rats,and to investigate the communicative ways between them.Methods Epilepsy models of 10 kindled rats established by intraperitoneal injection of PTZ[i.p.,35 mg/(kg · d)],assigned as kindled group.Five rats received 9 g/L saline as control group.Three days after being kindled,the ultrastructural relationship between neurons and the astrocytes was observed with transmission electron microscope and Cx43 labelling immuno-electron microscopy.Results 1.Synapses increased in hippocampi of PTZ-kindled epileptic rats.2.Gap junctions were observed between astrocytes and neurons.3.Astrocytic process extended into the synaptic cleft between pre-synaptic and post-synaptic membranes which formed the synaptic complex.4.The Cx43-hemichannels existed between astrocytes and neurons.Conclusions In hippocampi of PTZ-kindled epileptic rats,ultrastructure of morphological relationship between neurons and astrocytes includes synaptic complex,gap junctions and hemichannels,which might be communicative forms between neurons and astrocytes.
