Inhibitory effect of stomatin-like protein 2 (SLP-2) on growth of transplanted tumor of esophageal squamous cell carcinoma in nude mice
10.7507/1007-4848.201709036
- VernacularTitle:Stomatin 样蛋白2（SLP-2）抑制裸鼠食管鳞状细胞癌移植瘤生长的研究
- Author:
SONG Shuai
1
;
TIAN Di
2
;
CUI Yong
1
;
GAO Zhi
1
;
WANG Tianyou
1
;
HU Jian
1
;
CHANG Dong
1
Author Information
1. Department of Thoracic Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, P.R.China
2. Department of Infectious Diseases, China Meitan General Hospital, Beijing, 100028, P.R.China
- Publication Type:Journal Article
- Keywords:
Stomatin-like protein 2;
esophageal squamous cell carcinoma;
orthotopic implantation animal model;
in vivo imaging
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2018;25(10):885-889
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the growth of orthotopic transplanted tumor in nude mice after stomatin-like protein 2 (SLP-2) expression decreased, and to further study the role of SLP-2 in the development and progression of esophageal squamous cell carcinoma. Methods Using RNA interference technique, esophageal squamous cell carcinoma cell lines with specific expression of SLP-2 and stable expression of luciferase were established. The healthy female nude mice with weight ranging from 19 to 22 g were randomly divided into 3 groups (n=12), 6 mice were used to establish subcutaneous xenografts, and the other 6 mice were used to establish the orthotopic transplanted tumor model (Group 1: cell infected with SLP-2-1 plasmid; group 2: cell infected with SLP-2-2 plasmid; group 3: cell infected with SHGFP plasmid). Index of the experiment end was weight loss and poor general situation in any mouse. Before the nude mice were sacrificed, the luciferase value of the tumor was detected by using in vivo imaging technique. After the nude mice were sacrificed, the primary tumor was removed for pathology examination. Results There was no significant difference in region of interest (ROI) value between the group 1 and group 2 (P=0.943). The ROI value for both groups 1 and 2 was significantly lower than that in the group 3 (P=0.002, P=0.000). The primary tumor infiltrated into the muscularis propria of esophageal was observed in all groups. Conclusion SLP-2 is involved in the development and progression of esophageal squamous cell carcinoma, and the decrease of SLP-2 expression can inhibit the growth of esophageal squamous cell carcinoma.
