Effect of extracorporeal photochemotherapy on the regulatory T cells in mice with skin allograft Wei
10.3969/j.issn.1674-7445.2016.05.005
- VernacularTitle:体外光化学疗法对皮肤移植小鼠产生调节性T细胞的影响
- Author:
Yu-Xiang WEI
1
;
Shaozhen YANG
;
Li XIAO
;
Ming CAI
;
Yeyong QIAN
;
Tao YU
;
Bingyi SHI
Author Information
1. 解放军第309医院全军器官移植研究所 北京市器官移植与免疫调节重点实验室
- Keywords:
Extracorporeal photochemotherapy;
Skin allograft;
Mouse;
Regulatory T cell
- From:
Organ Transplantation
2016;7(5):355-359
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of infusion of spleen lymphocytes treated by extracorporeal photochemotherapy on the regulatory T cell (Treg)and survival time of skin allograft in mice. Methods The skin allograft model in mice was established with C57BL/6 mice as donors and BALB/c mice as recipients. The spleen lymphocytes (CSP,BSP)in mice C57BL/6 and BALB/c were isolated,and the mice spleen lymphocytes (PUVA-SP) treated with 8-methoxypsoralen plus ultraviolet (PUVA)were prepared. The experimental animals were randomly divided into 5 groups according to the compositions infused into the recipients through vein:PUVA-BSP,PUVA-CSP,BSP,CSP and phosphate buffer solution (PBS)control groups (n=12 in each group). All recipients of each group were injected with PUVA-BSP,PUVA-CSP,BSP,CSP or PBS on day 7 before the operation,on the operation day and day 7 after the operation through the tail vein,respectively. The survival time of graft in the recipients was observed,and the expression of CD4 +CD25 +Foxp3 +Treg in peripheral blood was detected. Results After skin allograft,the rate of CD4 +CD25 +Foxp3 +Treg in peripheral blood of the recipients in PUVA-BSP group and PUVA-CSP group was significantly higher than those of BSP, CSP and PBS control groups. The rate of CD4 +CD25 +Foxp3 +Treg in PUVA-CSP group was higher than that of PUVA-BSP group,while BSP and CSP groups were lower than that of PBS control group. The survival time of skin graft in the recipients in PUVA-BSP group and PUVA-CSP group was significantly longer than that of BSP,CSP and PBS control groups (all P<0.05 ). Conclusions Sufficient infusion of PUVA-SP can induce more CD4 +CD25 +Foxp3 +Treg in the recipients and prolong survival time of skin graft significantly.
