Autophagy of Human Tenon's Capsule Fibroblasts Induced by Mitomycin-C.
10.3341/jkos.2011.52.11.1337
- Author:
Channy PARK
1
;
Raekil PARK
;
Seong Kwang RYU
;
Dong Wook LEE
Author Information
1. Department of Audiology, Nambu University, Gwangju, Korea.
- Publication Type:Original Article
- Keywords:
Autophagy;
Fibroblast;
Mitomycin-C
- MeSH:
Acridine Orange;
Apoptosis;
Autophagy;
Cadaverine;
Coloring Agents;
Fibroblasts;
Flow Cytometry;
Humans;
Light;
Microscopy, Fluorescence;
Microtubule-Associated Proteins;
Mitomycin;
Organelles;
Phenotype;
Tenon Capsule
- From:Journal of the Korean Ophthalmological Society
2011;52(11):1337-1343
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The present study investigated whether an autophagic process is involved in the apoptotic death of human tenon's capsule fibroblasts (HTCFs) caused by mitomycin-C. METHODS: An autophagic phenotype was tested using fluorescence microscopy and flow cytometry with specific biological staining dyes including monodansylcadaverine and acridine orange and microtubule-associated protein 1 light chain 3 (LC3). RESULTS: Treatment with mitomycin-C (0.4 mg/ml) increased the acidic vesicular organelles of tenon's capsule fibroblasts in a time dependent manner. Mitomycin-C induced both LC3-II cleavage and beclin-1 expression. 3-MA, a pharmacological inhibitor of autophagy, inhibited the mitomycin-C induced increase of acidic vesicular organelleS. CONCLUSIONS: Autophagy was induced with 0.4 mg/ml mitomycin-C in tenon's capsule fibroblasts. And, autophagic mechanisms may be involved in the early stage of apoptosis of fibroblasts.
