Identification and Characterization of Eurotium rubrum Isolated from Meju in Korea.
10.4489/MYCO.2009.37.4.251
- Author:
Yeo Hong YUN
1
;
Min Woo HYUN
;
Dong Yeon SUH
;
Yong Min KIM
;
Seong Hwan KIM
Author Information
1. Department of Microbiology, Dankook University, Cheonan 330-714, Korea. piceae@naver.com
- Publication Type:Original Article
- Keywords:
beta-tubulin gene;
Calmodulin gene;
Eurotium rubrum;
ITS rDNA;
Meju
- MeSH:
beta-Glucosidase;
Calmodulin;
DNA, Ribosomal;
Eurotium;
Fungi;
Korea;
Light;
Spores, Fungal;
Sprains and Strains;
Tubulin
- From:Mycobiology
2009;37(4):251-257
- CountryRepublic of Korea
- Language:English
-
Abstract:
We isolated and identified a strain of Eurotium rubrum from Meju that has not been reported in Korea. This fungus is yellowish brown; reverse dark brown on CYA and PDA while yellow on 2% MEA at 25degrees C. Cleistothecia are first bright yellow and gradually turned brown. Mycerial growth on CYA attained a diameter of 30 mm at 20degrees C, 37 mm at 25degrees C and 32 mm at 30degrees C after 15 days. The isolate grew slower on 2% MEA (< 20 mm 15 days at 25degrees C) compared to CYA and PDA (< 40 mm 15 days at 25degrees C). Cleistothecia are superficial, yellow to light brown, globose to subglobose, 40~75 microm in diameter. Asci are 8-spored and globose to subglobose 8~11 microm. Ascospores are disciform, 4.0~5.0 microm in length and 4.2~4.5 microm in width. Conidia are ovate or bacillar, finely roughened to densely spinulose, 4.6~6.0 microm in length and 3.0~4.3 microm in width. Compared to known Eurotium rubrum, the Korean isolate showed 99% sequence similarity in ITS rDNA (554 bp) and calmodulin (750 bp) gene and 100% in beta-tubulin (1016 bp) gene. The E. rubrum isolate also had weak beta-glucosidase and protease activities.
