Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris.
- Author:
Nguyen Duc HUY
1
;
Saravanakumar THIYAGARAJAN
;
Yu Lim SON
;
Seung Moon PARK
Author Information
1. Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea. smpark@chonbuk.ac.kr
- Publication Type:Brief Communication
- Keywords:
Hemicellulose;
Phanerochaete chsysosporium;
Pichia pastoris;
Xylan;
Xylanase
- MeSH:
Chrysosporium;
DNA, Complementary;
Phanerochaete;
Pichia;
Polysaccharides;
Protein Sorting Signals;
Saccharomyces cerevisiae
- From:Mycobiology
2011;39(2):121-124
- CountryRepublic of Korea
- Language:English
-
Abstract:
The cDNA of endo-1,4-beta-xylanaseA, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the alpha-factor signal peptide of Saccharomyces cerevisiae, xylanaseA is efficiently secreted into the medium at maximum concentrations of 1,946 U/L and 2,496 U/L, respectively.
