Genomic Organization of ancop Gene for alpha-COP Homolog from Aspergillus nidulans.
- Author:
Hwan Hee LEE
;
Shun Kee CHAE
;
Jeong Yoon KIM
;
Pil Jae MAENG
;
Hee Moon PARK
- Publication Type:Original Article
- Keywords:
Aspergillus nidulans;
alpha-COP;
Genomic structure;
Sequence homology
- MeSH:
Amino Acid Sequence;
Arabidopsis;
Aspergillus nidulans*;
Aspergillus*;
Base Sequence;
Clone Cells;
Coatomer Protein*;
DNA;
DNA, Complementary;
Drosophila;
Drosophila melanogaster;
Humans;
Introns;
Saccharomyces cerevisiae;
Sequence Homology;
Transcription Initiation Site
- From:Mycobiology
2000;28(4):171-176
- CountryRepublic of Korea
- Language:English
-
Abstract:
We have cloned a alpha-COP homolog, ancop, from Aspergillus nidulans by colony hybridization of chromosome specific library using alpha-COP homologous fragment as a probe. The probe DNA was amplified with degenerated primers designed by comparison of conserved region of the amino acid sequences of Saccharomyces cerevisiae alpha-COP, Homo sapiens HEP-COP, and Drosophila melanogaster alpha-COP. Full length cDNA clone was also amplified by RT-PCR. Comparison of genomic DNA sequence with cDNA sequence obtained by RT-PCR revealed 7 introns. Amino acid sequence similarity search of the anCop with other alpha-COPs gave an overall identity of 52% with S. cerevisiae, 47% with human and bovine, 45% with Drosophila and Arabidopsis . In upstream region from the transcription start site, a putative TATA and CAAT motif were also identified.
