Lipoxygenase Inhibitory Activity of Korean Indigenous Mushroom Extracts and Isolation of an Active Compound from Phellinus baumii.
- Author:
Seung Woong LEE
1
;
Ja Gyeong SONG
;
Byung Soon HWANG
;
Dae Won KIM
;
Yoon Ju LEE
;
E Eum WOO
;
Ji Yul KIM
;
In Kyoung LEE
;
Bong Sik YUN
Author Information
1. Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University, Iksan 570-752, Korea. bsyun@jbnu.ac.kr
- Publication Type:Brief Communication
- Keywords:
Inoscavin A;
Korean native mushroom;
Lipoxygenase inhibitor;
Phellinus baumii
- MeSH:
Agaricales*;
Inhibitory Concentration 50;
Lipoxygenase*;
Mass Screening;
Methanol
- From:Mycobiology
2014;42(2):185-188
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated a total of 335 samples of Korean native mushroom extracts as part of our lipoxygenase (LOX) inhibitor screening program. Among the mushroom-methanolic extracts we investigated, 35 exhibited an inhibitory activity greater than 30% against LOX at a concentration of 100 microg/mL. Especially, Collybia maculata, Tylopilus neofelleus, Strobilomyces confusus, Phellinus gilvus, P. linteus, P. baumii, and Inonotus mikadoi exhibited relatively potent LOX inhibitory activities of 73.3%, 51.6%, 52.4%, 66.7%, 59.5%, 100.0%, and 85.2%, respectively. Bioassay-guided fractionation led to the isolation of inoscavin A from the methanolic extract of P. baumii, which showed the most potent activity and was identified by spectroscopic methods. Specifically, inoscavin A exhibited potent LOX inhibitory activity with an IC50 value of 6.8 microM.
