Changes of Cytosolic Ca2+ under Metabolic Inhibition in Isolated Rat Ventricular Myocytes.
- Author:
Sunghyun KANG
1
;
Nari KIM
;
Hyun JOO
;
Jae Boum YOUM
;
Won Sun PARK
;
Mohamed WARDA
;
Hyungkyu KIM
;
Dang Van CUONG
;
Taeho KIM
;
Euiyong KIM
;
Jin HAN
Author Information
1. Mitochondrial Signaling Laboratory, Department of Physiology and Biophysics, College of Medicine, Cardiovascular & Metabolic Disease Center, Biohealth Products Research Center, Inje University, Busan 614-735, Korea. phyhanj@ijnc.inje. ac.kr
- Publication Type:Original Article
- Keywords:
Dinitrophenol;
Caffeine;
Cytosolic Ca2+;
Laser Scanning Confocal Microscopy;
Fluo-4 AM;
Mitochondria;
Ventricular myocytes;
NCX;
KB-R7943;
Rhod-2 AM;
TMRE
- MeSH:
Animals;
Caffeine;
Cytosol*;
Fluorescence;
Fluorescent Dyes;
Membrane Potential, Mitochondrial;
Microscopy, Confocal;
Mitochondria;
Muscle Cells*;
Rats*;
Ryanodine;
Sarcoplasmic Reticulum;
Thapsigargin
- From:The Korean Journal of Physiology and Pharmacology
2005;9(5):291-298
- CountryRepublic of Korea
- Language:English
-
Abstract:
To characterize cytosolic Ca2+ fluctuations under metabolic inhibition, rat ventricular myocytes were exposed to 200microM 2, 4-dinitrophenol (DNP), and mitochondrial Ca2+, mitochondrial membrane potential (delta psi m), and cytosolic Ca2+ were measured, using Rhod-2 AM, TMRE, and Fluo-4 AM fluorescent dyes, respectively, by Laser Scanning Confocal Microscopy (LSCM). Furthermore, the role of sarcolemmal Na+/Ca2+ exchange (NCX) in cytosolic Ca2+ efflux was studied in KB-R7943 and Na+-free normal Tyrode's solution (143 mM LiCl ). When DNP was applied to cells loaded with Fluo-4 AM, Fluo-4 AM fluorescence intensity initially increased by 70+/-10% within 70+/-10 s, and later by 400+/-200% at 850+/-46 s. Fluorescence intensity of both Rhod-2 AM and TMRE were initially decreased by DNP, coincident with the initial increase of Fluo-4 AM fluorescence intensity. When sarcoplasmic reticulum (SR) Ca2+ was depleted by 1microM thapsigargin plus 10microM ryanodine, the initial increase of Fluo-4 AM fluorescence intensity was unaffected, however, the subsequent progressive increase was abolished. KB-R7943 delayed both the first and the second phases of cytosolic Ca2+ overload, while Na+-free solution accelerated the second. The above results suggest that: 1) the initial rise in cytosolic Ca2+ under DNP results from mitochondrial depolarization; 2) the secondary increase is caused by progressive Ca2+ release from SR; 3) NCX plays an important role in transient cytosolic Ca2+ shifts under metabolic inhibition with DNP.
