Voltage-dependent Ca2+ Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach.
10.4196/kjpp.2008.12.6.323
- Author:
Young Chul KIM
1
;
Hikaru SUZUKI
;
Wen Xie XU
;
Hikaru HASHITANI
;
Woong CHOI
;
Hyo Yung YUN
;
Seon Mee PARK
;
Sei Jin YOUN
;
Sang Jeon LEE
;
Sang Jin LEE
Author Information
1. Department of Physiology, Chungbuk National University, College of Medicine, Cheongju 361-763, Korea.
- Publication Type:Original Article
- Keywords:
Gastrointestinal (GI) tract;
Stomach;
Interstitial cells of Cajal (ICC);
Voltage-dependent Ca2+ current (VDCC)
- MeSH:
Interstitial Cells of Cajal;
Nifedipine;
Stomach
- From:The Korean Journal of Physiology and Pharmacology
2008;12(6):323-330
- CountryRepublic of Korea
- Language:English
-
Abstract:
The properties of voltage dependent Ca2+ current (VDCC) were investigated in interstitial cells of Cajal (ICC) distributed in the myenteric layer (ICC-MY) of guinea-pig antrum. In tissue, ICC-MY showed c-Kit positive reactions and produced driving potentials with the amplitude and frequency of about 62 mV and 2 times min(-1), respectively, in the presence of 1micrometer nifedipine. Single ICC-MY isolated by enzyme treatment also showed c-Kit immunohistochemical reactivity. These cells were also identified by generation of spontaneous inward current under K+-rich pipette solution. The voltage clamp experiments revealed the amplitude of - 329 pA inward current at irregular frequency. With Cs+-rich pipette solution at Vh=?80 mV, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine (1micrometer) blocked VDIC. Therefore, we successfully isolated c-Kit positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent Ca2+ currents (VDCCL).
