Betulin suppressed interleukin-1β-induced gene expression, secretion and proteolytic activity of matrix metalloproteinase in cultured articular chondrocytes and production of matrix metalloproteinase in the knee joint of rat.
10.4196/kjpp.2017.21.1.19
- Author:
Ho Jong RA
1
;
Hyun Jae LEE
;
Ho Seung JO
;
Dae Cheol NAM
;
Young Bok LEE
;
Byeong Hun KANG
;
Dong Kyu MOON
;
Dong Hee KIM
;
Choong Jae LEE
;
Sun Chul HWANG
Author Information
1. Department of Orthopedic Surgery, Gangneung Asan Hospital, College of Medicine, University of Ulsan, Gangneung 25440, Korea.
- Publication Type:Original Article
- Keywords:
Betulin;
Chondrocyte;
Metalloproteinase;
Osteoarthritis
- MeSH:
Animals;
Blotting, Western;
Caseins;
Chondrocytes*;
Collagen Type II;
Gene Expression*;
Knee Joint*;
Knee*;
Osteoarthritis;
Rats*;
Thrombospondins
- From:The Korean Journal of Physiology and Pharmacology
2017;21(1):19-26
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated whether betulin affects the gene expression, secretion and proteolytic activity of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as in vivo production of MMP-3 in the rat knee joint to evaluate the potential chondroprotective effect of betulin. Rabbit articular chondrocytes were cultured and reverse transcription-polymerase chain reaction (RT-PCR) was used to measure interleukin-1β (IL-1β)-induced gene expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), ADAMTS-5 and type II collagen. Effect of betulin on IL-1β-induced secretion and proteolytic activity of MMP-3 was investigated using western blot analysis and casein zymography, respectively. Effect of betulin on MMP-3 protein production was also examined in vivo. The results were as follows: (1) betulin inhibited the gene expression of MMP-3, MMP-1, MMP-13, ADAMTS-4, and ADAMTS-5, but increased the gene expression of type II collagen; (2) betulin inhibited the secretion and proteolytic activity of MMP-3; (3) betulin suppressed the production of MMP-3 protein in vivo. These results suggest that betulin can regulate the gene expression, secretion, and proteolytic activity of MMP-3, by directly acting on articular chondrocytes.
