Induction of Heat Shock Proteins and Antioxidant Enzymes in 2,3,7,8-TCDD-Induced Hepatotoxicity in Rats.
10.4196/kjpp.2012.16.6.469
- Author:
Hyun Sook KIM
1
;
So Young PARK
;
Ki Yeol YOO
;
Seung Kwan LEE
;
Woon Won JUNG
Author Information
1. Department of Biomedical Science, College of Health Science, Korea University, Seoul 136-703, Korea. woonun@korea.ac.kr
- Publication Type:Original Article
- Keywords:
2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD);
Antioxidant enzymes;
Gene expression;
Heat shock proteins;
Real-time PCR
- MeSH:
Animals;
Catalase;
Gene Expression;
Heat-Shock Proteins;
Hot Temperature;
HSP70 Heat-Shock Proteins;
Liver;
Rats;
Real-Time Polymerase Chain Reaction;
Tetrachlorodibenzodioxin
- From:The Korean Journal of Physiology and Pharmacology
2012;16(6):469-476
- CountryRepublic of Korea
- Language:English
-
Abstract:
2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) is an environmental toxicant with a polyhalogenated aromatic hydrocarbon structure and is one of the most toxic man-made chemicals. Exposure to 2,3,7,8-TCDD induces reproductive toxicity, immunotoxicity, and hepatotoxicity. In this study, we evaluated how 2,3,7,8-TCDD-induced hepatotoxicity affect the expression of heat shock proteins and antioxidant enzymes using the real-time polymerase chain reaction (PCR) in rat. 2,3,7,8-TCDD increased heat shock protein (Hsp27, alpha-B-crystallin, Mortalin, Hsp105, and Hsp90s) and antioxidant enzymes (SOD-3, GST and catalase) expression after a 1 day exposure in livers of rats, whereas heat shock protein (alpha-B-crystallin, Hsp90, and GRP78) and antioxidant enzymes (SOD-1, SOD-3, catalase, GST, and GPXs) expression decreased on day 2 and then slowly recovered back to control levels on day 8. These results suggest that heat shock proteins and antioxidant enzymes were induced as protective mechanisms against 2,3,7,8-TCDD induced hepatotoxicity, and that prolonged exposure depressed their levels, which recovered to control levels due to reduced 2,3,7,8-TCDD induced hepatotoxicity.
