Immunostimulatory Effects of beta-glucan Purified from Paenibacillus polymyxa JB115 on Mouse Splenocytes.
10.4196/kjpp.2012.16.4.225
- Author:
Ji Mi KIM
1
;
Hong Gu JOO
Author Information
1. Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea. jooh@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
beta-glucan;
Anti-apoptosis;
IL-2 dependency;
Splenocytes;
Viability
- MeSH:
Animals;
Cell Death;
Dependency (Psychology);
Diminazene;
Enzyme-Linked Immunosorbent Assay;
Interleukin-2;
Interleukin-7;
Lymphocytes;
Mice;
Paenibacillus;
Plasmodiophorida;
Trypan Blue
- From:The Korean Journal of Physiology and Pharmacology
2012;16(4):225-230
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated the effects of beta-glucan purified from Paenibacillus polymyxa JB115 on the viability and proliferation of splenocytes. Splenocytes play a critical role in host immunity. MTT assays and trypan blue exclusion tests revealed that beta-glucan significantly promoted the viability and proliferation of splenocytes over a range of concentrations. However, there was no specific subset change. beta-glucan protected splenocytes from cytokine withdrawal-induced spontaneous cell death. For further mechanistic studies, ELISA assay revealed that beta-glucan enhanced the expression of anti-apoptotic molecules and interleukin 7 (IL-7), a cytokine critical for lymphocyte survival. We also investigated the IL-2 dependency of beta-glucan-treated splenocytes to determine if treated cells could still undergo clonal expansion. In flow cytometric analysis, beta-glucan induced increased levels of the activation marker CD25 on the surface of splenocytes and beta-glucan-treated splenocytes showed higher proliferation rates in response to IL-2 treatment. This study demonstrates that beta-glucan can enhance the survival of splenocytes and provides valuable information to broaden the use of beta-glucan in research fields.
