Regulation of 3HNorepinephrine Release by Opioids in Human Cerebral Cortex.
- Author:
Ran Sook WOO
1
;
Byoung Soo SHIN
;
Chul Jin KIM
;
Min Soo SHIN
;
Min Suk JEONG
;
Rong Jie ZHAO
;
Kee Won KIM
Author Information
1. Department of Pharmacology, Chonbuk National University Medical School, Chonju, Chonbuk 561-180, Korea. keewon@moak.chon buk.ac.kr
- Publication Type:Original Article
- Keywords:
Opioid receptors;
Norepinephrine release;
Human cerebral cortex
- MeSH:
Analgesics, Opioid*;
Cerebral Cortex*;
Enkephalin, D-Penicillamine (2,5)-;
Humans*;
Naloxone;
Negotiating;
Norepinephrine;
Potassium;
Receptors, Opioid
- From:The Korean Journal of Physiology and Pharmacology
2003;7(1):1-3
- CountryRepublic of Korea
- Language:English
-
Abstract:
To investigate the receptors mediating the regulation of norepinephrine (NE) release in human cerebral cortex slices, we examined the effects of opioid agonists for mu-, delta-, and kappa -receptors on the high potassium (15 mM) -evoked release of [3H]NE. [3H]NE release induced by high potassium was calcium-dependent and tetrodotoxin-sensitive. [D-Pen2, D-Pen5]enkephalin (DPDPE) and deltorphin II (Delt II) inhibited the stimulated release of norepinephrine in a dose-dependent manner. However, Tyr-D-Ala-Gly- (Me) Phe-Gly-ol and U69, 593 did not influence the NE release. Inhibitory effect of DPDPE and Delt-II was antagonized by naloxone, naltrindole, 7-benzylidenaltrexone and naltriben. These results suggest that both delta 1 and delta 2 receptors are involved in regulation of NE release in human cerebral cortex.
