Naringin Protects against Rotenone-induced Apoptosis in Human Neuroblastoma SH-SY5Y Cells.
10.4196/kjpp.2009.13.4.281
- Author:
Hak Jae KIM
1
;
Jeong Yoon SONG
;
Hae Jeong PARK
;
Hyun Kyung PARK
;
Dong Hwan YUN
;
Joo Ho CHUNG
Author Information
1. Department of Pharmacology and Kohwang Medical Research Institute, School of Medicine, Kyung Hee University, Seoul 130-701, Korea. jhchung@khu.ac.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
Naringin;
Parkinson's disease;
Rotenone;
SH-SY5Y
- MeSH:
Apoptosis;
B-Lymphocytes;
bcl-2-Associated X Protein;
Caspase 3;
Caspase 9;
Cell Death;
Citrus paradisi;
Flavanones;
Humans;
Indoles;
Neuroblastoma;
Neuroprotective Agents;
Parkinson Disease;
Phosphorylation;
Protein Kinases;
Rotenone;
Tetrazolium Salts;
Thiazoles
- From:The Korean Journal of Physiology and Pharmacology
2009;13(4):281-285
- CountryRepublic of Korea
- Language:English
-
Abstract:
Rotenone, a mitochondrial complex I inhibitor, can induce the pathological features of Parkinson's disease (PD). In the present study, naringin, a grapefruit flavonoid, inhibited rotenone-induced cell death in human neuroblastoma SH-SY5Y cells. We assessed cell death and apoptosis by measuring mitogen-activated protein kinase (MAPKs) and caspase (CASPs) activities and by performing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. Naringin also blocked rotenone-induced phosphorylation of Jun NH2-terminal protein kinase (JNK) and P38, and prevented changes in B-cell CLL/lymphoma 2 (BCL2) and BCL2-associated X protein (BAX) expression levels. In addition, naringin reduced the enzyme activity of caspase 3 and cleavages of caspase 9, poly (ADP-ribose) polymerase (PARP), and caspase 3. These results suggest that naringin has a neuroprotective effect on rotenone-induced cell death in human neuroblastoma SH-SY5Y cells.
