Effect of Modulation of hnRNP L Levels on the Decay of bcl-2 mRNA in MCF-7 Cells.
10.4196/kjpp.2010.14.1.15
- Author:
Mi Hyun LIM
1
;
Dong Hyoung LEE
;
Seung Eun JUNG
;
Dong Ye YOUN
;
Chan Sun PARK
;
Jeong Hwa LEE
Author Information
1. Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea. leejh@catholic.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
hnRNP L;
bcl-2 mRNA stability
- MeSH:
3' Untranslated Regions;
Apoptosis;
Autophagy;
Breast;
Dactinomycin;
Hand;
Heterogeneous-Nuclear Ribonucleoprotein L;
Heterogeneous-Nuclear Ribonucleoproteins;
Humans;
MCF-7 Cells;
Phosphoproteins;
Ribonucleoproteins;
RNA, Messenger;
RNA, Small Interfering;
RNA-Binding Proteins
- From:The Korean Journal of Physiology and Pharmacology
2010;14(1):15-20
- CountryRepublic of Korea
- Language:English
-
Abstract:
It has been shown that CA repeats in the 3'-untranslated region (UTR) of bcl-2 mRNA contribute the constitutive decay of bcl-2 mRNA and that hnRNP L (heterogenous nuclear ribonucleoprotein L) interacts with CA repeats in the 3'-UTR of bcl-2 mRNA, both in vitro and in vivo. The aim of this study was to determine whether the alteration of hnRNP L affects the stability of bcl-2 mRNA in vivo. Human breast carcinoma MCF-7 cells were transfected with hnRNP L-specific shRNA or hnRNP L-expressing vector to decrease or increase hnRNP L levels, respectively, followed by an actinomycin D chase. An RT-PCR analysis showed that the rate of degradation of endogenous bcl-2 mRNA was not affected by the decrease or increase in the hnRNP L levels. Furthermore, during apoptosis or autophagy, in which bcl-2 expression has been reported to decrease, no difference in the degradation of bcl-2 mRNA was observed between control and hnRNP L-knock down MCF-7 Cells. On the other hand, the levels of AUF-1 and nucleolin, transacting factors for ARE in the 3'UTR of bcl-2 mRNA, were not significantly affected by the decrease in hnRNP L, suggesting that a disturbance in the quantitative balance between these transacting factors is not likely to interfere with the effect of hnRNP L. Collectively, the findings indicate that the decay of bcl-2 mRNA does not appear to be directly controlled by hnRNP L in vivo.
