The Effects of Human Adipose Tissue Derived Mesenchymal Stem Cells on Degenerative Change of Disc in Rabbit Model.
- Author:
Sang Beom KIM
1
;
Hyun KWAK
;
Kisung YOON
;
Kyeong Woo LEE
;
Ji Hoon PARK
;
Yong Seok KWON
;
Jin Yeong HAN
;
Jin Sook JEONG
;
Jong Hwa LEE
Author Information
1. Department of Physical Medicine and Rehabilitation, Dong-A University College of Medicine, Korea. rm0007@hanafos.com
- Publication Type:Original Article
- Keywords:
Transplanted human adipose tissue derived stem cells;
Disc degeneration;
Rabbit
- MeSH:
Actins;
Adipose Tissue*;
Hope;
Humans*;
Intervertebral Disc;
Intervertebral Disc Degeneration;
Mesenchymal Stromal Cells*;
Models, Theoretical;
Polymerase Chain Reaction;
Proteoglycans;
Rabbits;
Stem Cells;
Tissue Donors
- From:Journal of the Korean Academy of Rehabilitation Medicine
2007;31(3):269-277
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To determine whether transplanted human adipose tissue derived stem cells (hATSCs) can survive and increase the amount of proteoglycans in degenerated intervertebral disc. METHOD: Lumbar disc degeneration was induced in thirty New Zealand white rabbits by injection of chondroitinase ABC(R). After 2 weeks, hATSCs were transplanted in degenerated disc in hATSCs group. Control group received phosphate buffered saline. The histologic grading and height of disc were measured at 2, 4, and 8 weeks after transplantation. The viability of donor cells was identified by using beta-Actin gene polymerase chain reaction (PCR). RESULTS: 4 and 8 weeks after hATSCs transplantation, the histologic grading showed significantly high score in hATSCs group (p<0.05), but the amount of proteoglycans was not significantly different between the two groups. The change of disc height was not significantly increased in hATSCs group. In the beta-Actin gene PCR analysis, positive signal in the hATSCs group was observed. CONCLUSION: hATSCs transplantation may be useful in decelerating disc degeneration in experimental models and provide new hopes for treatment of degenerative disc disease in humans
