Killer Cell Immunoglobulin-like Receptor (KIR) Analysis in Adult Korean Patients with Acute Myeloid Leukemia.
10.5045/kjh.2006.41.3.139
- Author:
Hee Je KIM
1
;
Young CHOI
;
Hye Young JEONG
;
Woo Sung MIN
;
Chun Choo KIM
;
Tai Gyu KIM
Author Information
1. Department of Hematology-oncology, St. Mary's Hospital, The Catholic University of Korea College of Medicine, Seoul, Korea. cumckim@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
NK cells alloreactivity;
KIR;
HSCT;
Korean AML;
Haplotype
- MeSH:
Adult*;
Epitopes;
Genotype;
Graft vs Host Disease;
Haplotypes;
Hematopoietic Stem Cells;
Humans;
Killer Cells, Natural;
Leukemia, Myeloid, Acute*;
Polymerase Chain Reaction;
Pseudogenes;
Receptors, KIR*;
Siblings;
Tissue Donors
- From:Korean Journal of Hematology
2006;41(3):139-148
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The prevalent natural killer (NK) cells induce alloreaction against leukemic cells during post-transplant. NK cell alloreactivity depends on the compatibility of killer cell immunoglobulin-like receptors (KIR) epitopes for graft-versus-host disease. Genotypic expressions of inhibitory or activating KIR in patients with acute myelogenous leukemia (AML) and their HLA-matched sibling donors, as a model for Korean KIR haplotype diversity and NK alloreactivity, were investigated. METHODS: Ninety-two patients in complete remission and their 76 HLA-matched sibling donors were enrolled in this study. All the patients were scheduled to receive allogeneic hematopoietic stem cell transplantations (HSCT). KIR PCR-SSP typing was performed for 19 different kinds of KIR genes and pseudogenes. The PCR data representing the KIR genotypes from both the patients and donors were compared. RESULTS: We found 43 Korean KIR haplotypes. Thirty-three variable haplotypes for the AML patients, in addition to 25 haplotypes for the normal HSCT donors, were demonstrated. Of note, the expressions of specific genes such as 2DL2 (P=0.026), 2DS2 (P=0.042), and 2DS4 (P=0.037) revealed remarkable differences between the patients and the normal donors. Korean HLA-identical sibling pairs showed 38% KIR matches in terms of the gene content and allelic polymorphism. Although the KIR gene content was the same between the patients and the donors, 40% of those matched pairs of patients and donors showed allelic polymorphism, specifically in the context of 2DL5 and 2DS4 genes. CONCLUSION: These results indicate that the expressions of donor inhibitory and activating repertoire of KIR genotypes, even in the HLA-matched sibling setting, are unique parameters to be considered when we perform allogeneic sibling HSCT.
