Up-Regulation of MiR-1915 Inhibits Proliferation, Invasion, and Migration of Helicobacter pylori-Infected Gastric Cancer Cells via Targeting RAGE.
- Author:
Xin cai XU
1
;
Wen bin ZHANG
;
Chun xing LI
;
Hua GAO
;
Qi PEI
;
Bo wei CAO
;
Tie han HE
Author Information
- Publication Type:Original Article
- Keywords: Gastric cancer; microRNA-1915; MKN45; receptor for advanced glycation end product; SGC-7901
- MeSH: Blotting, Western; Cell Line; Cell Proliferation; Computational Biology; Gastritis; Helicobacter pylori; Helicobacter*; Humans; Luciferases; Rage*; Real-Time Polymerase Chain Reaction; Stomach Neoplasms*; Up-Regulation*
- From:Yonsei Medical Journal 2019;60(1):38-47
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: Helicobacter pylori (HP)-infected gastric cancer (GC) is known to be a fatal malignant tumor, but the molecular mechanisms underlying its proliferation, invasion, and migration remain far from being completely understood. Our aim in this study was to explore miR-1915 expression and its molecular mechanisms in regulating proliferation, invasion, and migration of HP-infected GC cells. MATERIALS AND METHODS: Quantitative real-time PCR and western blot analysis were performed to determine miR-1915 and receptor for advanced glycation end product (RAGE) expression in HP-infected GC tissues and gastritis tissues, as well as human gastric mucosal cell line GES-1 and human GC cell lines SGC-7901 and MKN45. CCK8 assay and transwell assay were performed to detect the proliferation, invasion, and migration capabilities. MiR-1915 mimics and miR-1915 inhibitor were transfected into GC cells to determine the target relationship between miR-1915 and RAGE. RESULTS: MiR-1915 was under-expressed, while RAGE was over-expressed in HP-infected GC tissues and GC cells. Over-expressed miR-1915 could attenuate cellular proliferation, invasion, and migration capacities. RAGE was confirmed to be the target gene of miR-1915 by bioinformatics analysis and luciferase reporter assay. Moreover, HP-infected GC cellular proliferation, invasion, and migration were inhibited after treatment with pcDNA-RAGE. CONCLUSION: MiR-1915 exerted tumor-suppressive effects on cellular proliferation, invasion, and migration of HP-infected GC cells via targeting RAGE, which provided an innovative target candidate for treatment of HP-infected GC.
