Dosage-Related Prebiotic Effects of Inulin in Formula-Fed Infants.
10.5223/pghn.2019.22.1.63
- Author:
Hanifah OSWARI
1
;
Ariani Dewi WIDODO
;
Frieda HANDAYANI
;
Mohammad JUFFRIE
;
Tonny SUNDJAYA
;
Jacques BINDELS
;
Badriul HEGAR
Author Information
1. Department of Child Health, Cipto Mangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia. hoswari@gmail.com
- Publication Type:Clinical Trial ; Randomized Controlled Trial ; Original Article
- Keywords:
Infant formula;
Prebiotics;
Inulin;
Gastrointestinal microbiome
- MeSH:
Dataset;
Gastrointestinal Microbiome;
Humans;
Hydrogen-Ion Concentration;
Infant Formula;
Infant*;
Inulin*;
Polymerase Chain Reaction;
Prebiotics*
- From:Pediatric Gastroenterology, Hepatology & Nutrition
2019;22(1):63-71
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The aim of this study was to identify the minimally meaningful dosage of inulin leading to a prebiotic effect in Indonesian infants. METHODS: In a randomized controlled double-blinded, parallel, 3-arm intervention study, 164 healthy formula-fed infants aged 3 to 5 months first obtained formula-A (without inulin) during a 4-week adaptation period. Subsequently, 142 subjects were subjected to a 4-week feeding period by administering either formula-A (no inulin), formula-B (0.2 g/100 mL inulin) or formula-C (0.4 g/100 mL inulin). The primary outcome parameter was %-bifidobacteria in faecal samples determined using quantitative polymerase chain reaction analyses. Secondary outcome parameters were faecal %-lactobacilli, pH and stool frequency, and consistency. Growth and tolerance/adverse effects were recorded as safety parameters. RESULTS: Typical %-bifidobacteria and %-lactobacilli at the end of the adaptation period in the study population were 14% and 2%, respectively. For faecal pH, significant differences between formula groups A vs. C and A vs. B were found at the end of the intervention period. Testing for differences in faecal %-bifidobacteria and %-lactobacilli between groups was hampered by non-normal data set distributions; no statistically significant differences were obtained. Comparisons within groups revealed that only in formula group C, all the three relevant parameters exhibited a significant effect with an increase in faecal %-bifidobacteria and %-lactobacilli and a decrease in pH. CONCLUSION: A consistent prebiotic effect along with a decrease in pH and increase in %-bifidobacteria and %-lactobacilli was found only in the group administered 0.4 g inulin/100 mL.
