Evaluation of in vitro anti-oxidant and anti-inflammatory activities of Korean and Chinese Lonicera caerulea.
10.4162/nrp.2018.12.6.486
- Author:
You Suk LEE
1
;
Il Je CHO
;
Joo Wan KIM
;
Sun Kyoung LEE
;
Sae Kwang KU
;
Hae Jeung LEE
Author Information
1. Department of Food and Nutrition, College of BioNano Technology, Gachon University, 1342, Seongnam-daero, Sujeong-gu, Seongnam, Gyeonggi 13120, Korea. skysea@gachon.ac.kr, skysea1010@gmail.com
- Publication Type:Original Article
- Keywords:
Honeysuckle berry;
hepatoprotective effect
- MeSH:
Animals;
Anthocyanins;
Ascorbic Acid;
Asian Continental Ancestry Group*;
Catalytic Domain;
Cats;
Flavonoids;
Fruit;
Glutamate-Cysteine Ligase;
Heme Oxygenase-1;
Humans;
In Vitro Techniques*;
Lonicera*;
Luciferases;
Nitric Oxide;
Oxidoreductases;
Phenol;
RAW 264.7 Cells;
RNA, Messenger
- From:Nutrition Research and Practice
2018;12(6):486-493
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/OBJECTIVES: The honeysuckle berry (HB) contains ascorbic acid and phenolic components, especially anthocyanins, flavonoids, and low-molecular-weight phenolic acids. In order to examine the potential of HB as a hepatoprotective medicinal food, we evaluated the in vitro anti-oxidant and anti-inflammatory activities of Korean HB (HBK) and Chinese HB (HBC). MATERIALS/METHODS: Antioxidant and anti-inflammatory effects of the extracts were examined in HepG2 and RAW 264.7 cells, respectively. The anti-oxidant capacity was determined by DPPH, SOD, CAT, and ARE luciferase activities. The production of nitric oxide (NO) as an inflammatory marker was also evaluated. The Nrf2-mediated mRNA levels of heme oxygenase-1 (HO-1), NAD(P)H dehydrogenase [quinone] 1 (Nqo1), and glutamate-cysteine ligase catalytic subunit (Gclc) were measured. The concentrations of HB extracts used were 3, 10, 30, 100, and 300 µg/mL. RESULTS: The radical scavenging activity of all HB extracts increased in a concentration-dependent manner (P < 0.01 or P < 0.05). SOD (P < 0.05) and CAT (P < 0.01) activities were increased by treatment with 300 µg/mL of each HB extract, when compared to those in the control. NO production was observed in cells pretreated with 100 or 300 µg/mL of HBC and HBK (P < 0.01). Treatment with 300 µg/mL of HBC significantly increased Nqo1 (P < 0.01) and Gclc (P < 0.05) mRNA levels compared to those in the control. Treatment with 300 µg/mL of HBK (P < 0.05) and HBC (P < 0.01) also significantly increased the HO-1 mRNA level compared to that in the control. CONCLUSIONS: Thus, the Korean and Chinese HBs were found to possess favorable in vitro anti-oxidant and anti-inflammatory activities. Nrf2 and its related anti-oxidant genes were associated with both anti-oxidant and anti-inflammatory activities in HB-treated cells. Further studies are needed to confirm these in vivo effects.
