Removing Lipemia in Serum/Plasma Samples: A Multicenter Study.
10.3343/alm.2018.38.6.518
- Author:
María José CASTRO-CASTRO
1
;
Beatriz CANDÁS-ESTÉBANEZ
;
Margarita ESTEBAN-SALÁN
;
Pilar CALMARZA
;
Teresa ARROBAS-VELILLA
;
Carlos ROMERO-ROMÁN
;
Miguel POCOVÍ-MIERAS
;
José Angel AGUILAR-DORESTE
Author Information
1. Clinical Laboratory, Bellvitge University Hospital, Barcelona, Spain. mjcastro@bellvitgehospital.cat
- Publication Type:Multicenter Study ; Original Article
- Keywords:
Lipemia;
Interference;
Lipid removal method;
High-speed centrifugation;
LipoClear;
1,1,2-trichlorotrifluoroethane
- MeSH:
Alanine Transaminase;
Alkaline Phosphatase;
Aspartate Aminotransferases;
Bilirubin;
Calcium;
Centrifugation;
Creatinine;
Glucose;
Hyperlipidemias*;
Laboratories, Hospital;
Liquid-Liquid Extraction;
Methods;
Potassium;
Sodium;
Spain;
Transferases;
Ultracentrifugation;
Urea
- From:Annals of Laboratory Medicine
2018;38(6):518-523
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Lipemia, a significant source of analytical errors in clinical laboratory settings, should be removed prior to measuring biochemical parameters. We investigated whether lipemia in serum/plasma samples can be removed using a method that is easier and more practicable than ultracentrifugation, the current reference method. METHODS: Seven hospital laboratories in Spain participated in this study. We first compared the effectiveness of ultracentrifugation (108,200×g) and high-speed centrifugation (10,000×g for 15 minutes) in removing lipemia. Second, we compared high-speed centrifugation with two liquid-liquid extraction methods—LipoClear (StatSpin, Norwood, USA), and 1,1,2-trichlorotrifluoroethane (Merck, Darmstadt, Germany). We assessed 14 biochemical parameters: serum/plasma concentrations of sodium ion, potassium ion, chloride ion, glucose, total protein, albumin, creatinine, urea, alkaline phosphatase, gamma-glutamyl transferase, alanine aminotransferase, aspartate-aminotransferase, calcium, and bilirubin. We analyzed whether the differences between lipemia removal methods exceeded the limit for clinically significant interference (LCSI). RESULTS: When ultracentrifugation and high-speed centrifugation were compared, no parameter had a difference that exceeded the LCSI. When high-speed centrifugation was compared with the two liquid-liquid extraction methods, we found differences exceeding the LCSI in protein, calcium, and aspartate aminotransferase in the comparison with 1,1,2-trichlorotrifluoroethane, and in protein, albumin, and calcium in the comparison with LipoClear. Differences in other parameters did not exceed the LCSI. CONCLUSIONS: High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples. LipoClear and 1,1,2-trichlorotrifluoroethane are unsuitable as they interfere with the measurement of certain parameters.
