Role of Interleukin-10-Expressing B Cells on the Pathophysiology of Chronic Sinusitis.
10.3342/kjorl-hns.2017.01067
- Author:
Hee Sung PARK
1
;
Soo Kyoung PARK
;
Sun Hee YEON
;
Jun XU
;
Ki Sang RHA
;
Yong Min KIM
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, Research Institute of Medical Science, Chungnam National University School of Medicine, Daejeon, Korea. entkym@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
B-lymphocytes;
Interleukin-10;
Rhinitis;
Sinusitis
- MeSH:
B-Cell Activating Factor;
B-Lymphocytes*;
B-Lymphocytes, Regulatory;
Cytokines;
Eosinophils;
Humans;
Immunohistochemistry;
Interleukin-10;
Methods;
Mucous Membrane;
Nasal Mucosa;
Nasal Polyps;
Real-Time Polymerase Chain Reaction;
Rhinitis;
Sinusitis*
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2018;61(8):412-420
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Many kinds of inflammatory cells and cytokines are suggested to be related with pathophysiology of chronic rhinosinusitis with nasal polyp (CRSwNP), but its mechanism is not yet fully understood. In particular, little is known about the function and role of Interleukin-10 (IL-10) producing regulatory B cells. The aim of this study was to investigate the presence and function of B cells, especially IL-10-producing regulatory B cells, in the nasal polyp (NP) and nasal mucosa. SUBJECTS AND METHOD: Twenty patients with CRSwNP, 10 patients with chronic rhinosinusitis without nasal polyp (CRSsNP) and 10 control subjects were enrolled in this study. NP tissues and uncinate tissues were collected for analysis. Real-time polymerase chain reaction (RT-PCR), immunohistochemistry were performed to measure the expression levels of the selected inflammatory cytokines and inflammation-associated molecules. RESULTS: In the mucosal tissues of CRSsNP patients, the number of IL-10+ B cells was significantly lower than that of NP and control mucosa of CRSwNP patients. The number of IL-10+ B cells was significantly increased in the eosinophilic NP, non-eosinohilic NP, CRSsNP, and control groups. There was a significant positive correlation between the number of IL-10+ B cells and B cell activating factor (BAFF). CONCLUSION: The expression of IL-10+ B cells and BAFF was significantly increased in CRSwNP patients compared to CRSsNP patients and control mucosal tissues, and IL-10+ B cells were more significantly increased in eosinophilic tissues. These results indicate that regulatory B cells are involved in the pathophysiology of eosinophilic NP and that BAFF contributes to the production of regulatory B cells.
