Effect of isoflavone-enriched whole soy milk powder supplementation on bone metabolism in ovariectomized mice.
10.4162/nrp.2018.12.4.275
- Author:
So Mi KIM
1
;
Hyun Sook LEE
;
Jae In JUNG
;
Su Min LIM
;
Ji Hoon LIM
;
Wang Hyun HA
;
Chang Lae JEON
;
Jae Yong LEE
;
Eun Ji KIM
Author Information
1. Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon 24252, Korea. myej4@hallym.ac.kr
- Publication Type:Original Article
- Keywords:
Functional food;
soybeans;
isoflavones;
ovariectomy;
bone remodeling
- MeSH:
Acid Phosphatase;
Alkaline Phosphatase;
Animals;
Bone Density;
Bone Remodeling;
Calcium;
Diet;
Female;
Functional Food;
Hormone Replacement Therapy;
Humans;
Isoflavones;
Metabolism*;
Mice*;
Mice, Inbred ICR;
Osteocalcin;
Osteogenesis;
Osteoporosis, Postmenopausal;
Ovariectomy;
Procollagen;
Soy Milk*;
Soybeans
- From:Nutrition Research and Practice
2018;12(4):275-282
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND/OBJECTIVE: There is intense interest in soy isoflavone as a hormone replacement therapy for the prevention of postmenopausal osteoporosis. A new kind of isoflavone-enriched whole soy milk powder (I-WSM) containing more isoflavones than conventional whole soy milk powder was recently developed. The aim of this study was to investigate the effects of I-WSM on bone metabolism in ovariectomized mice. MATERIALS/METHODS: Sixty female ICR mice individually underwent ovariectomy (OVX) or a sham operation, and were randomized into six groups of 10 animals each as follows: Sham, OVX, OVX with 2% I-WSM diet, OVX with 10% I-WSM diet, OVX with 20% I-WSM diet, and OVX with 20% WSM diet. After an 8-week treatment period, bone mineral density (BMD), calcium, alkaline phosphatase (ALP), tartrate-resistant acid phosphatase (TRAP) 5b, osteocalcin (OC), procollagen 1 N-terminal propeptide (P1NP), and osteoprotegenin (OPG) were analyzed. RESULTS: BMD was significantly lower in the OVX group compared to the Sham group but was significantly higher in OVX + 10% I-WSM and OVX + 20% I-WSM groups compared to the OVX group (P < 0.05). Serum calcium concentration significantly increased in the OVX + 10% and 20% I-WSM groups. Serum ALP levels were significantly lower in the OVX + 10% and 20% I-WSM groups compared to the other experimental groups (P < 0.05). OC was significantly reduced in the OVX group compared to the Sham group (P < 0.05), but a dose-dependent increase was observed in the OVX groups supplemented with I-WSM. P1NP and OPG levels were significantly reduced, while TRAP 5b level was significantly elevated in the OVX group compared with the Sham group, which was not affected by I-WSM (P < 0.05). CONCLUSIONS: This study suggests that I-WSM supplementation in OVX mice has the effect of preventing BMD reduction and promoting bone formation. Therefore, I-WSM can be used as an effective alternative to postmenopausal osteoporosis prevention.
