Effects of the Helicobacter pylori Virulence Factor CagA and Ammonium Ion on Mucins in AGS Cells.
10.3349/ymj.2018.59.5.633
- Author:
Xiaoyu ZHANG
1
;
Ding SHI
;
Yong pan LIU
;
Wu jie CHEN
;
Dong WU
Author Information
1. Department of Gastroenterology, Henan University of Chinese Medicine, Zhengzhou, China.
- Publication Type:Original Article
- Keywords:
Mucin;
Helicobacter pylori;
CagA;
ammonium chloride;
stomach
- MeSH:
Ammonium Chloride;
Ammonium Compounds*;
Cadherins;
Fluorescent Antibody Technique;
Helicobacter pylori*;
Helicobacter*;
Mucins*;
RNA, Messenger;
Stomach;
Urease;
Virulence*
- From:Yonsei Medical Journal
2018;59(5):633-642
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: To investigate the effects of Helicobacter pylori (H. pylori)-CagA and the urease metabolite NH₄⁺ on mucin expression in AGS cells. MATERIALS AND METHODS: AGS cells were transfected with CagA and/or treated with different concentrations of NH₄⁺CL. Mucin gene and protein expression was assessed by qPCR and immunofluorescence assays, respectively. RESULTS: CagA significantly upregulated MUC5AC, MUC2, and MUC5B expression in AGS cells, but did not affect E-cadherin and MUC6 expression. MUC5AC, MUC6, and MUC2 expression in AGS cells increased with increasing NH₄⁺ concentrations until reaching a peak level at 15 mM. MUC5B mRNA expression in AGS cells (NH₄⁺ concentration of 15 mM) was significantly higher than that at 0, 5, and 10 mM NH₄⁺. No changes in E-cadherin expression in AGS cells treated with NH₄⁺ were noted, except at 20 mM. The expression of MUC5AC, MUC2, and MUC6 mRNA in CagA-transfected AGS cells at an NH₄⁺ concentration of 15 mM was significantly NH₄⁺ concentration, and was significantly higher compared to that in untreated cells. No significant change in the expression of E-cadherin mRNA in CagA-transfected AGS cells was observed. Immunofluorescence assays confirmed the observed changes. CONCLUSION: H. pylori may affect the expression of MUC5AC, MUC2, MUC5B, and MUC6 in AGS cells via CagA and/or NH₄⁺, but not E-cadherin.
