14-bp Insertion/Deletion Polymorphism of the HLA-G Gene in Osteosarcoma Patients.
10.4132/KoreanJPathol.2011.45.5.485
- Author:
Ahrim MOON
1
;
Su Kang KIM
;
Joo Ho CHUNG
;
Ki Yong NA
;
Liliana G OLVI
;
Eduardo SANTINI-ARAUJO
;
Youn Wha KIM
;
Yong Koo PARK
Author Information
1. Department of Pathology, School of Medicine, Kyung Hee University, Seoul, Korea. ykpark@khmc.or.kr
- Publication Type:Original Article
- Keywords:
HLA-G;
14-bp insertion/deletion polymorphism;
Osteosarcoma
- MeSH:
DNA;
Exons;
Genotype;
Heterozygote;
HLA-G Antigens;
Humans;
Leukocytes;
Major Histocompatibility Complex;
Organ Transplantation;
Osteosarcoma;
Transplants
- From:Korean Journal of Pathology
2011;45(5):485-490
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The major histocompatibility complex class I, G (human leukocyte antigen-G [HLA-G]) gene plays a vital role in the suppression of immune responses. Recently, a number of studies have reported an association between HLA-G and diseases (pregnancy complications, organ transplantation, and tumors). Some of the studies have revealed that the 14-bp insertion/deletion polymorphism might be associated with various diseases. The aim of the present study was to explore a possible influence of the 14-bp insertion/deletion polymorphism on osteosarcoma. METHODS: Genomic DNA was extracted from 75 formalin-fixed, paraffin-embedded tumor tissues derived from patients with conventional osteosarcoma (OSA) and 183 peripheral blood samples of healthy controls. Fifty-eight cases were South Korean patients with OSA and 17 cases were Argentine patients with OSA. The HLA-G 14-bp insertion/deletion polymorphism at exon 8 of the HLA-G locus was analyzed by polymerase chain reaction. RESULTS: There was a significantly different distribution profile for the 14-bp genotypes between the Korean OSA and Korean control groups. Specifically, there were more heterozygote 210 bp/224 bp genotypes in the Korean OSA group when compared to the Korean control group (62.1% vs 40.4%, p=0.002). CONCLUSIONS: The results suggest that HLA-G heterozygote patients may be more susceptible to OSA in the Korean population.
