Analysis of Peripheral B Cell Subsets in Patients With Allergic Rhinitis.
10.4168/aair.2018.10.3.236
- Author:
Jing LUO
1
;
Huanhuan GUO
;
Zhuofu LIU
;
Tao PENG
;
Xianting HU
;
Miaomiao HAN
;
Xiangping YANG
;
Xuhong ZHOU
;
Huabin LI
Author Information
1. Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
- Publication Type:Original Article
- Keywords:
Allergic rhinitis;
flow cytometry;
B cell subsets;
regulatory B cells;
immunoglobulin E
- MeSH:
B-Lymphocyte Subsets*;
B-Lymphocytes;
B-Lymphocytes, Regulatory;
Enzyme-Linked Immunosorbent Assay;
Flow Cytometry;
Humans;
Immunoglobulin E;
Immunoglobulins;
Memory;
Plasma Cells;
Rhinitis, Allergic*
- From:Allergy, Asthma & Immunology Research
2018;10(3):236-243
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Recent evidence suggests that B cells can both promote and inhibit the development and progression of allergic disease. However, the characteristics of B cell subsets in patients with allergic rhinitis (AR) have not been well documented. This study aimed to analyze the characteristics of B cell subsets in the peripheral blood of AR patients. METHODS: Forty-seven AR patients and 54 healthy controls were enrolled in this study, and the B cell subsets in peripheral blood of all subjects were analyzed by flow cytometry. Moreover, the serum total immunoglobulin E (IgE) and IgE concentrations secreted into the cultured peripheral blood mononuclear cells (PBMCs) were measured by using enzyme-linked immunosorbent assay. RESULTS: We found the peripheral blood of AR patients contained higher percentages of memory B cells, plasma cells, and CD19+CD24hiCD27+ regulatory B cells (Bregs) than those of age-matched healthy controls (P < 0.05), while the percentages of naïve B cells and CD19+CD24hiCD38hi Bregs were significantly lower in AR patients than in healthy individuals (P < 0.05). In addition, the serum total IgE and IgE concentrations secreted into the cultured PBMCs were elevated in AR patients than in the healthy controls (P < 0.05). CONCLUSIONS: Our findings indicate that AR patients were characterized by increase in terminally differentiated memory B cells or plasma cells and decreases in CD19+CD24hiCD38hi Breg cells in the peripheral blood.
