Alginate-Catechol Cross-Linking Interferes with Insulin Secretion Capacity in Isolated Murine Islet Cells.
10.4093/dmj.2018.42.2.164
- Author:
Yu Sik KIM
1
;
Seung Woo CHO
;
Bomin KO
;
Jisoo SHIN
;
Chul Woo AHN
Author Information
1. Severance Institute for Vascular and Metabolic Research, Yonsei University College of Medicine, Seoul, Korea. acw@yuhs.ac
- Publication Type:Brief Communication
- Keywords:
Alginic acid;
Catechol;
Diabetes mellitus, type 1;
Hydrogel;
Islets of Langerhans transplantation
- MeSH:
Architectural Accessibility;
Diabetes Mellitus, Type 1;
Humans;
Hydrogel;
Hydrogen-Ion Concentration;
Immunosuppression;
Insulin*;
Islets of Langerhans Transplantation;
Islets of Langerhans*;
Temefos;
Tissue Donors
- From:Diabetes & Metabolism Journal
2018;42(2):164-168
- CountryRepublic of Korea
- Language:English
-
Abstract:
Over the past three decades, human pancreatic islet isolation and transplantation techniques have developed as a routine clinical procedure for selected patients with type 1 diabetes mellitus. However, due to the donor shortage and required chronic systemic immunosuppression, the widespread application of islet transplantation is limited. To overcome these limitations, providing a physical barrier to transplanted islet cells with encapsulating biomaterial has emerged as a promising approach to enhance engraftment and promote islet survival post-transplantation. Alginate has been considered to be a reliable biomaterial, as it enhances islet survival and does not hamper hormone secretion. Alginate-catechol (Al-CA) hydrogel was reported to provide high mechanical strength and chemical stability without deformation over a wide range of pH values. In this study, we, demonstrated, for the first time in the literature, that encapsulation of murine pancreatic islet cells with Al-CA hydrogel does not induce cytotoxicity ex vivo for an extended period; however, it does markedly abate glucose-stimulated insulin secretion. Catechol should not be considered as a constituent for alginate gelation for encapsulating islet cells in the application of islet transplantation.
